Anti-CYP2D6 Rabbit Polyclonal Antibody for WB and ELISA - P10635

Core Product Specifications and Parameters

Biological Background: CYP2D6 Function and Localization

• CYP2D6 is a member of the cytochrome P450 superfamily, functioning as a versatile monooxygenase that requires NADPH and molecular oxygen to catalyze the oxidation of numerous endogenous and xenobiotic substrates (PubMed:18698000, PubMed:19965576, PubMed:20972997). Related references: PMID:18698000 PMID:19965576 PMID:20972997
• It metabolizes polyunsaturated fatty acids (PUFAs) through epoxidation and converts endocannabinoids like anandamide into signaling lipids such as 20-HETE-EA and EpETrE-EAs, thereby modulating endocannabinoid system activity (PubMed:18698000, PubMed:21289075). Related references: PMID:18698000 PMID:21289075
• CYP2D6 participates in cholesterol homeostasis by catalyzing the conversion of cholesterol to 25-hydroxycholesterol, a key regulatory molecule (PubMed:21576599). Related references: PMID:21576599
• In retinoid metabolism, it oxidizes all-trans retinol to all-trans retinal, a critical step in the generation of active retinoic acid (PubMed:10681376). Related references: PMID:10681376
• CYP2D6 is critically involved in the oxidative metabolism of numerous drugs, including antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants.
• The enzyme is anchored to the endoplasmic reticulum and microsomal membranes, indicative of its role in membrane-bound metabolic processes.
• Alternative splicing yields multiple transcript variants encoding different isoforms, and the protein contains a heme iron cofactor essential for monooxygenase activity; it belongs to pathways of lipid, steroid, and fatty acid metabolism.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 20–230 near the N-terminus; consider verifying that this region is exposed in native membrane fractions during WB.
• For Western blot applications, a band around 56 kDa is expected. Membrane protein extraction and sample preparation may influence band migration; validate under reducing and non-reducing conditions as needed.
• The antibody has demonstrated cross-reactivity with mouse and rat samples, making it suitable for comparative studies. However, we recommend confirming reactivity in your specific tissue or cell lysates.
• For ELISA, titration against recombinant CYP2D6 protein or cell lysates expressing the target is advised to determine optimal working concentrations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive sourcing solution for IVD raw materials, and this anti-CYP2D6 polyclonal antibody is part of our extensive portfolio for drug metabolism and cytochrome P450 research. Beyond individual antibodies, we offer validated antibody pairs, monoclonal options, and bulk ancillary reagents, along with tailored sourcing support for rare or difficult targets. Partner with us to streamline your diagnostic development from early research to clinical application.