Anti-CYP2D6 Rabbit Monoclonal Antibody for WB, IF-P, ELISA - P10635

Core Product Specifications and Parameters

Biological Background: CYP2D6 Function and Localization

• Cytochrome P450 2D6 (CYP2D6), also known as debrisoquine 4-hydroxylase, is a monooxygenase enzyme belonging to the cytochrome P450 superfamily. It is encoded by the CYP2D6 gene, which undergoes alternative splicing to generate multiple isoforms.
• CYP2D6 catalyzes the oxidative metabolism of diverse endogenous and exogenous substrates, including fatty acids, steroids, retinoids, and numerous therapeutic drugs (e.g., antiarrhythmics, adrenoceptor antagonists, tricyclic antidepressants). Related references: PMID:18698000 PMID:19965576 PMID:20972997
• The enzyme is primarily localized to the membrane of the endoplasmic reticulum and microsomal fractions of hepatic cells, consistent with its central role in phase I metabolism.
• CYP2D6 contains a heme-iron cofactor essential for its catalytic activity and is classified as a metal-binding oxidoreductase.
• It specifically metabolizes endocannabinoids, such as anandamide, producing epoxy- and hydroxy-derivatives that may modulate signaling pathways. Related references: PMID:18698000 PMID:21289075
• CYP2D6 also converts cholesterol to 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. Related references: PMID:21576599
• Additionally, it catalyzes the oxidative transformation of all-trans retinol to all-trans retinal, a precursor for retinoic acid biosynthesis. Related references: PMID:10681376

Experimental Guidance and Technical Tips

• For Western blotting (WB), a band of approximately 56 kDa is expected. Use appropriate positive control lysates from human, mouse, or rat liver tissue or cells expressing CYP2D6 to confirm specificity.
• In immunofluorescence-paraffin (IF-P) experiments, optimize antigen retrieval and permeabilization steps to ensure adequate exposure of the endoplasmic reticulum membrane-localized target. Counterstaining with an ER marker is recommended for colocalization.
• For ELISA applications, consider using the recombinant CYP2D6 immunogen fragment as a quantitative standard to generate reliable calibration curves.
• Due to genetic polymorphisms in CYP2D6, expression levels may vary across individuals; it is advisable to validate the antibody in the intended sample matrix and consider genotypic information when interpreting results.
• The monoclonal rabbit source ensures high affinity and reproducibility across batches, but optimal dilutions should be empirically determined for each assay and sample type.

CamelBio: Your One-Stop Sourcing Bridge

As a comprehensive IVD raw material partner, CamelBio connects diagnostic manufacturers and research laboratories with high-quality reagents, technical expertise, and supply chain solutions—from initial concept to clinical application. This CYP2D6 Rabbit mAb exemplifies our commitment to providing validated, application-ready antibodies critical for pharmacogenomics, drug metabolism, and diagnostic assay development. Beyond antibodies, we offer customized sourcing of ancillary reagents, bulk-scale monoclonal and polyclonal antibody pairs, and rare-target raw materials to streamline your development pipeline and reduce time-to-market.