Anti-CYP2C18 Rabbit Polyclonal Antibody for WB, ELISA - P33260

Core Product Specifications and Parameters

Biological Background: CYP2C18 Function and Localization

• Cytochrome P450 2C18 (CYP2C18) is a member of the cytochrome P450 superfamily, specifically the 2C subfamily, and also known as CYPIIC18 or Cytochrome P450-6b/29c.
• It functions as a monooxygenase that hydroxylates all-trans-retinoic acid (atRA) to 4-hydroxyretinoate, playing a role in retinoid metabolism and the modulation of atRA signaling and clearance.
• The enzyme incorporates one atom from molecular oxygen into its substrate and reduces the second atom to water, using electrons supplied by NADPH via cytochrome P450 reductase (CPR).
• CYP2C18 localizes to the endoplasmic reticulum membrane and microsome membrane, characteristic of many cytochromes P450 involved in lipid metabolism.
• It contains a heme prosthetic group and an iron cofactor, which are essential for its monooxygenase activity.
• Alternative splicing of the CYP2C18 gene produces multiple transcript variants, potentially giving rise to isoforms with distinct functions.
• The protein is associated with keywords including 3D-structure, lipid metabolism, oxidoreductase, and proteomics identification, reflecting its role as a reference proteome component.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 231-490 of human CYP2C18; thus, the antibody may recognize epitopes within this region, and full-length recombinant protein can serve as a positive control in ELISA.
• For Western blot, consider using subcellular fractions enriched in endoplasmic reticulum or microsomes (e.g., liver tissue lysates) to enhance detection of this ~56kDa membrane-associated protein. Validate in the relevant sample system.
• As the antibody shows cross-reactivity with mouse and rat, it may be suitable for studies in rodent models, but verify specificity using knockout tissues or siRNA knockdown if available.
• Due to the polyclonal nature, lot-to-lot variability may occur; we recommend routine quality control with a consistent positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with reliable access to IVD raw materials, including validated antibodies like this anti-CYP2C18 polyclonal antibody for cytochrome P450 and retinoid metabolism research. Our portfolio encompasses optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing, all backed by technical consulting to streamline your development from concept to clinic.