Anti-CYP27A1 Rabbit Monoclonal Antibody for WB and ELISA - Q02318

Core Product Specifications and Parameters

Biological Background: CYP27A1 (Sterol 26-hydroxylase) Function and Localization

• CYP27A1 encodes a mitochondrial cytochrome P450 enzyme, sterol 26‑hydroxylase, belonging to the CYP27 family.
• It catalyzes the three‑step hydroxylation of cholesterol at the terminal methyl group, producing 27‑hydroxycholesterol, a critical oxysterol.
  Related references: PMID:12077124, PMID:21411718, PMID:28190002
• Through this activity, CYP27A1 regulates cholesterol homeostasis by initiating bile acid synthesis via both the classic and alternative pathways.
  Related references: PMID:11412116, PMID:1708392, PMID:2019602
• The resulting oxysterols act as ligands for liver X receptors (LXRs), modulating the transcription of genes involved in lipid metabolism.
  Related references: PMID:12077124, PMID:9660774
• CYP27A1 is also involved in the metabolism of 7‑ketocholesterol in the retina, potentially protecting against inflammatory damage, and in cholestanol metabolism in the cerebellum, aiding sterol clearance across the blood–brain barrier.
  Related references: PMID:21411718, PMID:28190002
• Additionally, CYP27A1 may contribute to vitamin D activation by catalyzing the 25‑hydroxylation of vitamin D3.
  Related references: PMID:15465040
• The protein resides in the mitochondrial inner membrane and is detected in neural retina, retinal pigment epithelium, and cerebellar gray and white matter.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C‑terminal peptide (aa 431‑530); confirm antibody reactivity against both native and denatured CYP27A1 via parallel WB and ELISA assays.
• For Western blot, a band at ~60 kDa is expected; using mitochondrial‑enriched lysates from liver or retinal tissue may improve detection sensitivity.
• In ELISA, consider using a synthetic peptide or full‑length recombinant CYP27A1 as capture antigen to assess binding specificity and affinity.
• Cross‑reactivity with mouse and rat CYP27A1 makes this antibody suitable for translational studies, but validate reactivity in the relevant species‑specific sample matrices.
• Since CYP27A1 is a low‑abundance membrane protein, consider immunoprecipitation or signal amplification strategies for low‑expressing samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti‑CYP27A1 rabbit monoclonal antibody as part of our extensive IVD raw‑material portfolio for lipid metabolism and bile acid research. We supply validated antibody pairs, optimized recombinant antigens, and bulk ancillary reagents to streamline your diagnostic assay development from concept to clinic. Rely on CamelBio’s sourcing expertise and technical support to accelerate your cholesterol‑related biomarker projects.