Anti-CYP27A1 Polyclonal Antibody for WB, ELISA - Q02318

Core Product Specifications and Parameters

Biological Background: CYP27A1 Function and Localization

• Cytochrome P450 27A1 (CYP27A1), also known as sterol 26-hydroxylase or vitamin D3 25-hydroxylase, is a mitochondrial monooxygenase essential for cholesterol metabolism and bile acid synthesis.
• Catalyzes the regio- and stereospecific hydroxylation of cholesterol and its derivatives, converting the terminal methyl group to a carboxylate in a three-step reaction; this step is critical in both the classic and alternative bile acid pathways. Related references:
  • PMID:11412116
  • PMID:1708392
  • PMID:2019602
• Generates active oxysterol ligands, such as 27-hydroxycholesterol, that act on NR1H2 and NR1H3 nuclear receptors to regulate lipid metabolism gene transcription. Related references:
  • PMID:12077124
  • PMID:9660774
• Hydroxylates 7-ketocholesterol to form 7-keto,27-hydroxycholesterol, which can activate the smoothened (SMO) signaling pathway (By similarity).
• Mediates cholestanol hydroxylation in the cerebellum, facilitating sterol diffusion through the blood-brain barrier and systemic clearance. Related references:
  • PMID:28190002
• Contributes to vitamin D3 activation via 25-hydroxylation, a redox step required for full hormonal activity. Related references:
  • PMID:15465040
• Localized to the mitochondrial inner membrane, consistent with its role in sterol processing.
• Expressed in neural retina, retinal pigment epithelium, and cerebellar gray/white matter at the protein level. Related references:
  • PMID:21411718
  • PMID:28190002
• Mutations in CYP27A1 cause cerebrotendinous xanthomatosis (CTX), a rare lipid storage disease affecting bile acid synthesis.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 200–400) covers a portion of the catalytic domain; ensure the antibody epitope is accessible under your experimental conditions.
• For Western blot, a band of approximately 60 kDa is expected; consider using mitochondrial-enriched lysates or whole-cell extracts from liver or retina as positive controls.
• Validate antibody specificity by comparing signal in CYP27A1‑overexpressing cells versus knockdown/knockout models.
• For ELISA, titrate the antibody using recombinant CYP27A1 or peptide antigen to determine the optimal working dilution.
• Cross‑reactivity with mouse and rat enables translational studies; however, verify cross‑reactivity in your specific tissue or cell line.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one‑stop access to premium IVD raw materials. Our CYP27A1 antibody portfolio supports investigations into cholesterol metabolism, bile acid disorders, and related diseases. Beyond antibodies, we offer validated antibody pairs, optimized monoclonal/polyclonal reagents, and bulk ancillary materials to accelerate your assay development from concept to clinic.