Anti-CYP1A2 Polyclonal Antibody for WB, IF-P, ELISA - P05177

Core Product Specifications and Parameters

Biological Background: CYP1A2 Function and Localization

• CYP1A2 is a cytochrome P450 monooxygenase that catalyzes the hydroxylation of carbon-hydrogen bonds, inserting one oxygen atom into substrates while reducing the second to water, with electrons provided by NADPH via cytochrome P450 reductase. Related references: PMID:10681376, PMID:11555828, PMID:12865317

• It exhibits high catalytic activity for the formation of hydroxyestrogens from estrone and 17beta-estradiol, playing a role in estrogen homeostasis. Related references: PMID:11555828, PMID:12865317

• CYP1A2 metabolizes cholesterol to 25-hydroxycholesterol, a physiological regulator of cellular cholesterol homeostasis. Related references: PMID:21576599

• It may act as a major enzyme for all-trans retinoic acid biosynthesis in the liver, catalyzing the conversion of all-trans retinol to all-trans retinal and then to retinoic acid. Related references: PMID:10681376

• Involved in the metabolism of polyunsaturated fatty acids (PUFAs), including stereoselective epoxidation and bisallylic hydroxylation. Related references: PMID:19965576, PMID:9435160

• Participates in xenobiotic metabolism by N-hydroxylating heterocyclic amines, O-deethylating phenacetin, and N3-demethylating caffeine. Related references: PMID:14725854

• Subcellularly localized to the endoplasmic reticulum membrane and microsomes, CYP1A2 is predominantly expressed in the liver.

• The protein is a glycoprotein and a heme-iron containing enzyme, as indicated by keywords including Glycoprotein, Heme, and Iron.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 205–305 of human CYP1A2. When using this antibody, consider that it may recognize epitopes within this region, and validate specificity with appropriate controls (e.g., knockdown or knockout lysates).
• For Western blotting, a band around 58 kDa is expected; ensure proper sample preparation and loading, especially when using liver-derived samples where CYP1A2 is enriched.
• In immunofluorescence (IF-P), anticipate signal primarily in the endoplasmic reticulum; co-staining with an ER marker is recommended.
• Although cross-reactivity is reported for mouse and rat, it is advisable to verify performance in your specific model system, as sequence homology within the immunogen region may vary.
• ELISA protocols should be optimized individually; consider testing a range of coating antigen concentrations and antibody dilutions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, supporting diagnostic manufacturers and research laboratories with high-quality reagents and technical services. This CYP1A2 antibody exemplifies our capability to supply validated antibodies for drug metabolism and toxicology research. In addition to polyclonal and monoclonal antibodies, CamelBio offers antibody pairs, bulk ancillary reagents, and customized sourcing for rare targets to accelerate your assay development from concept to clinic.