Anti-CYP1A1 Rabbit Polyclonal Antibody for WB, IF-P, IHC-P, ELISA - P04798

Core Product Specifications and Parameters

Biological Background: CYP1A1 Function and Localization

• CYP1A1 is a member of the cytochrome P450 superfamily, encoded by the CYP1A1 gene, and is also known as cytochrome P450 form 6, cytochrome P450-C, and cytochrome P450-P1.
• Functions as a monooxygenase that inserts one oxygen atom into substrates, using NADPH and cytochrome P450 reductase for electron transfer, and is involved in the metabolism of fatty acids, steroid hormones, and vitamins. Related references: PMID:10681376, PMID:11555828, PMID:12865317
• Catalyzes the hydroxylation of estrogens, converting estrone and 17beta-estradiol to 2-hydroxy and D-ring hydroxylated metabolites, thereby playing a role in hormone regulation. Related references: PMID:11555828, PMID:12865317, PMID:14559847
• Hydroxylates polyunsaturated fatty acids (PUFAs) with regioselectivity and epoxidates double bonds of PUFAs such as arachidonic acid to generate epoxyeicosatrienoic acids (EETs), which are lipid mediators in the vascular system. Related references: PMID:15041462, PMID:18577768, PMID:19965576, PMID:20972997
• Contributes to retinoic acid biosynthesis by oxidizing all-trans retinol to all-trans retinal and then to all-trans retinoic acid in extrahepatic tissues. Related references: PMID:10681376
• Exhibits lipoxygenase-like activity, converting hydroperoxide eicosanoids into oxo metabolites in an NADPH-independent manner. Related references: PMID:21068195
• Subcellularly localized primarily to the endoplasmic reticulum membrane, but also found in mitochondrial inner membrane, microsomes, and cytoplasm.
• Expressed in lung, lymphocytes, and placenta. It is a glycoprotein with heme and iron cofactors, and alternative splicing has been reported.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide spanning amino acids 261–360 of human CYP1A1, so the antibody is expected to recognize the total protein in denaturing and native applications.
• For Western blotting, an approximate band at 58 kDa should be observed. Validate by including positive controls such as lung or placenta lysates.
• For IHC-P and IF-P, consider optimizing antigen retrieval and validate staining in known expressing tissues (e.g., lung, placenta). Cross-reactivity with mouse and rat indicates a conserved epitope; however, always confirm specificity in the relevant experimental system.
• For ELISA, use appropriate peptide or recombinant protein standards and confirm linearity of detection.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research laboratories by supplying validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and rare-target raw materials. For CYP1A1-related projects in drug metabolism, endocrinology, or lipid signaling, our one-stop platform streamlines procurement—from initial inquiry to delivery—so you can focus on advancing your assays and product development.