Anti-CYP17A1 Rabbit Polyclonal Antibody for WB, ELISA - P05093

Core Product Specifications and Parameters

Biological Background: CYP17A1 Function and Localization

• CYP17A1 (Cytochrome P450 17A1), also known as P450c17, is a member of the cytochrome P450 superfamily encoded by the CYP17A1 gene (aliases: CYP17, S17AH, CPT7).
• Catalyzes both 17-alpha hydroxylase and 17,20 lyase reactions, essential for glucocorticoid and androgen biosynthesis. Converts progesterone and pregnenolone into 17-alpha hydroxy steroids, then cleaves the C17-C20 bond to produce androstenedione and DHEA. Related references: PMID:22266943 PMID:25301938 PMID:27339894
• Demonstrates additional 16-alpha hydroxylase activity, converting 17-alpha hydroxy pregnenolone to 16-alpha-hydroxy DHEA and hydroxylating androgens, contributing to estriol synthesis. Related references: PMID:25301938 PMID:27339894 PMID:36640554
• Subcellularly, CYP17A1 is localized to the endoplasmic reticulum membrane and microsomal membrane.
• Contains a heme iron cofactor and is classified as a monooxygenase/oxidoreductase; it is a phosphoprotein involved in steroid and lipid metabolism.
• Mutations in CYP17A1 are linked to congenital adrenal hyperplasia, a disorder of steroidogenesis.
• The full-length human CYP17A1 protein has a molecular weight of approximately 57kDa.

Experimental Guidance and Technical Tips

• The immunogen (amino acids 209–508 of human CYP17A1) covers part of the catalytic domain, which may confer target specificity.
• In WB, a band near 57kDa is anticipated; verify using positive controls such as adrenal or gonadal tissue lysates.
• Reported cross-reactivity with rat suggests potential utility in rodent models; confirm reactivity in your specific rat sample system.
• For ELISA applications, optimize antibody coating concentrations and consider its use as either capture or detection reagent.
• Because CYP17A1 is an endoplasmic reticulum membrane protein, microsomal or membrane-enriched fractions may improve detection.
• Phosphorylation may cause minor molecular weight shifts; consider phosphatase treatment if precise size confirmation is needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive portfolio of IVD antibodies and raw materials, including validated polyclonal and monoclonal antibodies, optimized antibody pairs, and bulk ancillary reagents for steroidogenesis research. This CYP17A1 antibody supports diagnostic development for adrenal and gonadal disorders, with flexible ordering and technical guidance available. From concept to clinic, CamelBio streamlines sourcing for rare and established targets.