Anti-CYP11B2 Rabbit Polyclonal Antibody for WB, IF-P, ELISA - P19099

Core Product Specifications and Parameters

| Product Name | CYP11B2 Rabbit pAb | | Remarks/Alias | CYP11B2; ALDOS; CPN2; CYP11B; CYP11BL; CYPXIB2; P-450C18; P450C18; P450aldo; cytochrome P450 11B2; mitochondrial | | Species | Human | | GeneID (Human) | 1585 | | GeneID | 1585 | | Immunogen | Synthetic peptide|A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CYP11B2(NP_000489.3). | | Source | Rabbit | | Category | Polyclonal Antibodies | | Application | WB, IF-P, ELISA | | Cross Reactivity | Human, Mouse, Rat | | SWISS | P19099 | | Protein Weight | 57kDa | | Shipping | Ice bag |

Biological Background: CYP11B2 Function and Localization

• Cytochrome P450 11B2, mitochondrial (aldosterone synthase, ALDOS) is a heme-containing monooxygenase involved in steroid hormone biosynthesis.
• Catalyzes the three-step conversion of 11-deoxycorticosterone to aldosterone, the main mineralocorticoid regulating salt and water homeostasis, blood pressure, and heart failure development. Related references: PMID:11856349 PMID:12530636 PMID:1518866
• Can also produce 18-hydroxycortisol and 18-oxocortisol from cortisol; levels are elevated in primary aldosteronism. Related references: PMID:15356073 PMID:9814482
• Electron transfer from NADPH occurs via adrenodoxin reductase (FDXR) and adrenodoxin (FDX1/FDX2). Related references: PMID:11856349 PMID:1594605 PMID:23322723
• Participates in androgen metabolism by converting testosterone to 11β,17β-dihydroxyandrost-4-ene-3-one. Related references: PMID:23685396
• Can use progesterone as a substrate. Related references: PMID:33189850
• Subcellular location: Mitochondrion inner membrane.
• Tissue specificity: Expressed sporadically in the zona glomerulosa of the adrenal cortex and in aldosterone-producing cell clusters.
• Keywords: Steroidogenesis, oxidoreductase, heme, iron, mitochondrial membrane, disease variant.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide from the N-terminal region (amino acids 1–100). This sequence may be exposed in denaturing conditions, making the antibody suitable for WB. For native detection, IF-P and ELISA may also be attempted, but validation in the relevant sample system is recommended.
• Given the high sequence homology among CYP11B family members, cross-reactivity with other cytochromes (e.g., CYP11B1) might occur. Include isoform-specific positive and negative controls to verify specificity.
• CYP11B2 is a mitochondrial membrane protein. For Western blotting, consider using mitochondrial enrichment or specific lysis buffers to improve detection of this 57 kDa protein.
• The antibody has been tested to cross-react with mouse and rat CYP11B2. When working with these species, validate the antibody performance under your experimental conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop sourcing partner for IVD raw materials, supporting diagnostic developers, laboratories, and research institutes from concept to clinic. We supply validated antibodies, including anti-CYP11B2 polyclonal antibodies for hypertension and steroidogenesis research. Our portfolio covers bulk reagents, ancillary materials, and customized sourcing for rare targets, ensuring seamless integration into your assay development pipeline.