Anti-CYP11B1 Polyclonal Antibody for IHC-P, IF/ICC, ELISA - P15538

Core Product Specifications and Parameters

Biological Background: CYP11B1 Function and Localization

• CYP11B1 is a mitochondrial cytochrome P450 monooxygenase that catalyzes the 11β-hydroxylation of 11-deoxycortisol and 11-deoxycorticosterone to produce cortisol and corticosterone, respectively, playing a central role in adrenal glucocorticoid and mineralocorticoid biosynthesis (PubMed:12530636, PubMed:1518866, PubMed:1775135). Related references: PMID:12530636, PMID:1518866, PMID:1775135
• The enzyme utilizes a mitochondrial electron transfer system involving NADPH, adrenodoxin reductase (FDXR), and adrenodoxin (FDX1/FDX2) to activate molecular oxygen for substrate hydroxylation (PubMed:18215163, PubMed:23685396). Related references: PMID:18215163, PMID:23685396
• CYP11B1 lacks 18-oxidation activity and therefore cannot produce aldosterone, distinguishing it from the closely related aldosterone synthase (CYP11B2) (PubMed:23322723). Related references: PMID:23322723
• The protein also participates in androgen metabolism, converting androstenedione to 11β-hydroxyandrostenedione and testosterone to 11β-hydroxytestosterone (PubMed:23685396, PubMed:29277707). Related references: PMID:23685396, PMID:29277707
• CYP11B1 is localized to the mitochondrial inner membrane, with expression primarily restricted to the zona fasciculata and zona reticularis of the adrenal cortex.
• Keywords associated with CYP11B1 include lipid biosynthesis, steroidogenesis, heme-binding, iron, and disease variants linked to congenital adrenal hyperplasia, reflecting its key roles in steroid hormone production and metabolic disorders.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 234-503 of human CYP11B1, a region within the cytochrome P450 domain; consider using peptide competition or knockout validation to confirm specificity in your sample system.
• For IHC-P applications, formalin-fixed paraffin-embedded adrenal tissue sections are recommended with appropriate antigen retrieval steps and inclusion of positive and negative controls.
• In IF/ICC, mitochondrial co-staining markers can help confirm subcellular localization consistent with mitochondrial inner membrane positioning.
• Optimal working dilutions and conditions should be empirically determined for each experimental setup, as performance can vary with sample type and preparation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive IVD raw material supply services, providing validated antibody reagents—including polyclonal and monoclonal options—to support research and diagnostic development in steroid hormone biology and adrenal function. Whether you need bulk ancillary reagents or rare-target sourcing for projects involving CYP11B1 and related pathways, our one-stop platform connects you with quality-assured materials from concept to commercialization. Let CamelBio streamline your procurement for reliable, research-use antibodies and technical consultation.