Anti-CYP11A1 Rabbit Polyclonal Antibody for WB, IHC-P, IF-P, ELISA - P05108

Core Product Specifications and Parameters

Biological Background: CYP11A1 Function and Localization

• CYP11A1 encodes cholesterol side-chain cleavage enzyme (P450scc), a mitochondrial cytochrome P450 monooxygenase that initiates steroidogenesis by converting cholesterol to pregnenolone.
  Related references: PMID:21636783
• The enzyme catalyzes three sequential oxidation reactions: hydroxylation at C22, then C20, followed by oxidative cleavage of the C20-C22 bond, releasing pregnenolone and 4-methylpentanal.
  Related references: PMID:21636783
• Electrons are supplied by NADPH through a mitochondrial transfer system involving adrenodoxin reductase (FDXR) and adrenodoxin (FDX1/FDX2).
  Related references: PMID:21636783
• CYP11A1 localizes to the mitochondrial inner membrane, a key compartment for its role in steroid hormone biosynthesis.
• The protein is a member of the cytochrome P450 superfamily, featuring a heme iron cofactor essential for monooxygenase activity.
• Alternative splicing generates multiple isoforms; pathogenic variants in CYP11A1 are associated with congenital adrenal hyperplasia and lipid biosynthesis disorders.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide within amino acids 40-320 of human CYP11A1. Given its polyclonal nature, expect robust signal but consider validating in relevant tissue or cell models.
• For Western blot, a band at ~60 kDa is predicted; use mitochondrial-enriched lysates from steroidogenic tissues (e.g., adrenal gland, testis) as positive control.
• In IHC-P and IF-P, staining is expected in mitochondria-rich cells; optimize antigen retrieval and permeabilization protocols accordingly.
• ELISA applications may require titration to determine optimal coating and detection conditions; pair with matched antibody pairs if intended for sandwich ELISA.
• Cross-reactivity is reported for human, mouse, and rat; sequence alignment with other species is advised before use in non-listed species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio empowers diagnostic developers with comprehensive access to IVD raw materials, technical services, and expert consulting from concept through clinical application. This anti-CYP11A1 polyclonal antibody supports research and assay development in steroidogenesis and endocrinology. We offer validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your IVD projects.