Anti-CYP11A1 Rabbit Monoclonal Antibody for WB and ELISA - P05108

Core Product Specifications and Parameters

Biological Background: CYP11A1 Function and Localization

• CYP11A1, also known as cholesterol side-chain cleavage enzyme or cytochrome P450 11A1, is a mitochondrial monooxygenase that initiates steroidogenesis by converting cholesterol to pregnenolone. Related references: PMID:21636783
• The enzyme catalyzes three sequential oxidation reactions: hydroxylation at C22, hydroxylation at C20, and cleavage of the C20-C22 bond to yield pregnenolone and 4-methylpentanal. Related references: PMID:21636783
• This process requires molecular oxygen and electrons transferred from NADPH via the mitochondrial electron transfer system comprising adrenodoxin reductase (FDXR) and ferredoxin (FDX1 or FDX2). Related references: PMID:21636783
• CYP11A1 is localized to the mitochondrial inner membrane, consistent with its role in cholesterol metabolism and steroid hormone biosynthesis.
• The gene undergoes alternative splicing and is associated with keywords such as Cholesterol metabolism, Lipid biosynthesis, Steroidogenesis, Monooxygenase, and Heme/Iron binding.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 40–320 of human CYP11A1, suggesting that the antibody may recognize both native and denatured forms of the protein; consider optimizing mitochondrial protein extraction methods for enriched tissue lysates.
• For Western blot, note that CYP11A1 has reported isoforms at approximately 42 kDa and 60 kDa; confirm the expected band pattern using appropriate molecular weight markers and validate with human or mouse positive controls.
• In ELISA applications, the recombinant immunogen can serve as a positive control to assess antibody binding; include relevant biological replicates to account for sample variability.
• Given the reported cross-reactivity with mouse, this antibody could be suitable for comparative studies across species, but always verify specificity in the target model system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials and technical support, from concept to clinic. This CYP11A1 rabbit monoclonal antibody supports research into steroidogenesis and related endocrine disorders. Whether you need validated antibody pairs, optimized recombinant proteins, or bulk ancillary reagents, CamelBio streamlines your sourcing for rare and in-demand targets.