Anti-CYLD Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - Q9NQC7

Core Product Specifications and Parameters

Biological Background: CYLD Function and Localization

• CYLD is a deubiquitinase that specifically cleaves 'Lys-63'- and linear 'Met-1'-linked polyubiquitin chains, playing a key role in NF-kappa-B activation and TNF-induced necroptosis.
  Related references: PMID:18313383, PMID:18636086, PMID:26670046

• Negatively regulates NF-kappa-B activation by deubiquitinating upstream signaling factors, contributing to the regulation of cell survival, proliferation, and differentiation.
  Related references: PMID:12917689, PMID:12917691, PMID:32185393, PMID:12917690

• Functions as a negative regulator of Wnt signaling.
  Related references: PMID:20227366

• Inhibits HDAC6, promoting acetylation of alpha-tubulin and stabilization of microtubules; thereby regulates microtubule dynamics, cell polarization, migration, and angiogenesis.
  Related references: PMID:19893491, PMID:18222923, PMID:20194890

• Required for normal cell cycle progression and cytokinesis.
  Related references: PMID:17495026, PMID:19893491

• Involved in innate immune regulation by restricting linear polyubiquitin formation on RIPK2 and RIPK1, and by modulating NLRP6 inflammasome activity.
  Related references: PMID:26670046, PMID:26997266, PMID:27458237

• Subcellular localization includes cytoplasm, perinuclear region, cytoskeleton, cell membrane, centrosome, spindle, and cilium basal body.

• Detected in fetal brain, testis, and skeletal muscle; at lower levels in adult brain, leukocytes, liver, heart, kidney, spleen, ovary, and lung. Isoform 2 is found in all tissues except kidney.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 654–953, a region containing part of the USP catalytic domain; this design may confer cross-reactivity across human, mouse, and rat due to sequence conservation.
• For Western blotting, the predicted band is around 107 kDa; ensure adequate gel percentage and transfer conditions for high molecular weight proteins.
• For immunohistochemistry (IHC-P), both nuclear and cytoplasmic staining may be observed, consistent with CYLD localization; optimize antigen retrieval and antibody dilution using appropriate positive control tissues.
• For ELISA, use the recombinant immunogen or target protein for coating and evaluate detection linearity.
• When working with mouse or rat samples, cross-reactivity data suggest applicability; however, validate in the specific tissue or cell context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes a comprehensive one-stop platform for sourcing IVD raw materials. For CYLD and related NF-κB/Wnt signaling targets, we can supply validated antibody pairs, optimized monoclonal and polyclonal antibodies like this product, and bulk ancillary reagents. Our expertise extends to rare target raw-material sourcing, helping accelerate your assay development and diagnostic projects.