Anti-CYLD Polyclonal Antibody for IF/ICC, IF-P, ELISA - Q9NQC7

Core Product Specifications and Parameters

Biological Background: CYLD Function and Localization

• Functions as a deubiquitinase that specifically cleaves 'Lys-63'-linked and linear 'Met-1'-linked polyubiquitin chains. Related references: PMID:18313383, PMID:18636086, PMID:26670046
• Negatively regulates NF-κB signaling by deubiquitinating upstream factors, thereby modulating cell survival, proliferation, and differentiation. Related references: PMID:12917689, PMID:12917691, PMID:32185393, PMID:12917690
• Acts as a negative regulator of the Wnt signaling pathway. Related references: PMID:20227366
• Inhibits HDAC6 to promote α-tubulin acetylation and microtubule stabilization, contributing to cell polarization, migration, and angiogenesis. Related references: PMID:19893491, PMID:18222923, PMID:20194890
• Essential for normal cell cycle progression and cytokinesis. Related references: PMID:17495026, PMID:19893491
• Recruited to the LUBAC complex via SPATA2 to restrict linear ubiquitination on RIPK2 and other targets, thereby regulating innate immune responses and TNF-induced necroptosis. Related references: PMID:26670046, PMID:26997266, PMID:27458237
• Localizes to the cytoplasm, cytoskeleton, centrosome, spindle, and cilium basal body, in line with its roles in microtubule dynamics and cell cycle control.
• Expressed in fetal brain, testis, and skeletal muscle, and at lower levels in adult brain, leukocytes, liver, heart, kidney, spleen, ovary, and lung; isoform 2 is absent in kidney.

Experimental Guidance and Technical Tips

• The immunogen comprising the N-terminal region (aa 1–127) may generate antibodies recognizing the N-terminus of CYLD; validate specificity in your target species and experimental system.
• The antibody is supplied for IF/ICC, IF-P, and ELISA applications; consider optimizing dilution, fixation, and blocking conditions for your specific sample type.
• Cross-reactivity with human and mouse is indicated; when working with other species, verify reactivity experimentally.
• For functional studies involving ubiquitination or signaling, pair with appropriate controls (e.g., deubiquitinase inhibitors or knockdown/knockout validation).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, supporting every stage from concept to clinic. For CYLD and related NF-κB/ubiquitin pathway targets, we offer validated antibody pairs, optimized polyclonal and monoclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Partner with us to streamline your diagnostic development and accelerate assay readiness.