Anti-CYFIP1 Polyclonal Antibody for WB, IP, ELISA - Q7L576

Core Product Specifications and Parameters

Biological Background: CYFIP1 Function and Localization

• CYFIP1 (Cytoplasmic FMR1-interacting protein 1) is also known as Specifically Rac1-associated protein 1 (Sra-1) or p140sra-1, and is encoded by the CYFIP1 gene (KIAA0068) in humans.
• It functions as a key adapter in the CYFIP1-EIF4E-FMR1 complex, mediating translational repression by linking the cap-binding protein EIF4E to the fragile X mental retardation protein FMR1, particularly in the brain.
• CYFIP1 is a core component of the WAVE regulatory complex, which activates the Arp2/3 complex to drive actin filament reorganization and lamellipodia formation, regulated by the small GTPase RAC1.
• It binds directly to F-actin, contributing to membrane ruffle dynamics and cell shape changes, and plays a role in axon outgrowth and neurogenesis.
• Subcellular localization includes the cytoplasm, perinuclear region, lamellipodia, ruffles, and synaptosomes, reflecting its roles in both cytoskeletal and synaptic functions.
• CYFIP1 may act as an invasion suppressor in cancers and is involved in epithelial morphogenesis, highlighting its importance in both developmental and pathological contexts.
• Post-translational modifications include phosphorylation, and the protein undergoes alternative splicing, generating multiple isoforms.
• Keywords: 3D-structure, Actin-binding, Alternative splicing, Cell projection, Cell shape, Cytoplasm, Developmental protein, Differentiation, Neurogenesis, Phosphoprotein, Synapse, Synaptosome.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was generated against an N-terminal fragment (aa 1-270) of human CYFIP1. For western blotting, the full-length protein has a predicted molecular weight of ~145 kDa; confirm signal specificity using appropriate controls.
• When performing immunoprecipitation, consider using a validated detection antibody targeting a different epitope to avoid heavy/light chain interference, and optimize lysis conditions to preserve CYFIP1 interactions.
• This antibody recognizes human and mouse CYFIP1; for other species, verify sequence homology in the immunogen region before use.
• ELISA applications may benefit from using recombinant protein as a standard to ensure linear detection range, given the immunogen is a recombinant fusion protein.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to IVD raw materials and technical support, from concept to clinic. For CYFIP1-related research in neurodevelopment, cytoskeletal dynamics, and cancer biology, we offer validated antibody pairs, bulk monoclonal/polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets. Partner with CamelBio to accelerate your assay development and scale-up with confidence.