Anti-Cyclophilin B Monoclonal Antibody for WB, ELISA - P23284

Core Product Specifications and Parameters

Biological Background: Cyclophilin B Function and Localization

• Peptidyl-prolyl cis-trans isomerase B (PPIB), also known as Cyclophilin B (CYPB), rotamase B, or S-cyclophilin (SCYLP), is encoded by the PPIB gene on human chromosome 15.
• Catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, a rate-limiting step in protein folding and trafficking.
• Functions as a molecular chaperone within the endoplasmic reticulum (ER), aiding proper folding and secretion of proteins.
• Subcellular localization includes the endoplasmic reticulum lumen, melanosomes, and virions, mediated by an N-terminal signal sequence.
• Post-translational modifications include acetylation, S-nitrosylation, and glycosylation, as indicated by UniProt keywords.
• Mutations in PPIB are associated with osteogenesis imperfecta and other skeletal dysplasias, highlighting its role in collagen processing.
• The cyclophilin family shares a conserved peptidyl-prolyl isomerase domain; Cyclophilin B also exhibits chemotactic activity for leukocytes.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 100–200 of human Cyclophilin B; consider this region for epitope mapping or blocking experiments.
• For WB, recommended sample preparation includes reducing conditions due to potential disulfide bonds in the ER environment.
• Although predicted to react with mouse and rat, validate specificity in your sample system using positive and negative controls.
• In ELISA, the antibody may be used as capture or detection reagent; optimal working concentrations should be determined by titration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers, laboratories, and research institutes with a comprehensive portfolio of IVD raw materials, technical services, and consulting—covering the entire journey from concept to clinic. This Cyclophilin B monoclonal antibody is a key tool for exploring protein folding disorders, ER stress pathways, and collagen-related diseases; we can complement it with validated antibody pairs, bulk ancillary reagents, and rare-target raw-material sourcing to streamline your assay development. Reach out for tailored procurement solutions and expert technical guidance.