Anti-Cyclophilin 40 Polyclonal Antibody for WB, IF/ICC, ELISA - Q08752

Core Product Specifications and Parameters

Biological Background: Cyclophilin 40 Function and Localization

• Cyclophilin 40, encoded by the PPID gene, is a 40 kDa peptidyl-prolyl cis-trans isomerase D (CYP-40), belonging to the cyclophilin family.
• Functions as a peptidyl-prolyl cis-trans isomerase (PPIase), catalyzing the cis-trans isomerization of proline imidic peptide bonds in oligopeptides, thereby assisting protein folding.
  Related references: PMID:11350175 PMID:20676357
• Acts as a co-chaperone in HSP90 complexes, particularly in unligated steroid receptor heterocomplexes, with a preference for estrogen receptor complexes; different co-chaperones compete for HSP90 binding, establishing tissue-specific receptor activity control.
• Localizes to the cytoplasm, nucleus, nucleolus, and nucleoplasm, consistent with roles in protein folding and receptor transport.
• Widely expressed across tissues, with potential involvement in cytoplasmic dynein-dependent receptor movement from the cytoplasm to the nucleus.
• Regulates MYB by inhibiting its DNA-binding activity, and participates in AHR signaling by promoting AHR:ARNT dimer formation, independently of HSP90.
• Involved in regulation of UV radiation-induced apoptosis and promotes cell viability in ALK+ ALCL cell lines; undergoes post-translational modifications including acetylation and phosphorylation.
• May be involved in hepatitis C virus (HCV) replication and release, highlighting a role in host-virus interactions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 161-370 of human Cyclophilin 40 (NP_005029.1), covering the C-terminal region; the antibody may detect both full-length and truncated forms.
• For Western blotting, consider using denaturing/reducing conditions with appropriate loading controls; the predicted molecular weight is approximately 41 kDa, but confirm in relevant sample systems.
• In immunofluorescence, validate the antibody in cells or tissues known to express Cyclophilin 40, and consider co-staining with compartment-specific markers to assess localization.
• ELISA performance may require optimization of coating concentrations and blocking conditions; include appropriate positive and negative controls.
• Cross-reactivity with mouse and rat is reported; when using these species, verify band patterns and consider sequence homology in the target region.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, including validated antibody pairs, optimized polyclonal and monoclonal antibodies against targets like Cyclophilin 40. Our comprehensive supply chain covers bulk ancillary reagents and rare target raw-material sourcing, supporting your projects from concept to clinic in areas such as protein folding, steroid receptor signaling, and viral replication.