Anti-Cyclophilin 40 Polyclonal Antibody for WB, ELISA - Q08752

Core Product Specifications and Parameters

Biological Background: Cyclophilin 40 Function and Localization

• Cyclophilin 40, officially designated peptidyl-prolyl cis-trans isomerase D (PPID), is also known as 40 kDa peptidyl-prolyl cis-trans isomerase, CYP-40, and rotamase D. It catalyzes the cis-trans isomerization of proline imidic peptide bonds, a critical step in protein folding.
• As a PPIase, PPID facilitates protein folding by accelerating the isomerization of proline bonds in oligopeptides. Related references: PMID:11350175 PMID:20676357
• PPID acts as a co-chaperone within HSP90 complexes, particularly in unligated steroid receptor heterocomplexes. It shows preference for estrogen receptor complexes and may influence receptor trafficking via cytoplasmic dynein-dependent movement to the nucleus.
• Beyond its chaperone role, PPID inhibits the DNA-binding activity of MYB, regulates AHR signaling by promoting AHR:ARNT dimer formation independently of HSP90, and modulates UV radiation-induced apoptosis. It also promotes cell viability in ALK+ anaplastic large-cell lymphoma (ALCL) cell lines.
• PPID may be involved in the replication and release of hepatitis C virus (HCV).
• Subcellularly, PPID localizes to the cytoplasm, nucleus, nucleolus, and nucleoplasm.
• PPID is widely expressed across tissues.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains TPR repeats characteristic of immunophilins.

Experimental Guidance and Technical Tips

• For Western blotting, use appropriate loading controls and validate the expected band size of approximately 41 kDa. Since the antibody is polyclonal and cross-reacts with rat, consider validating in your specific sample system.
• In ELISA, the antibody can be used as a detection or capture reagent depending on your assay design. Optimize dilution factors empirically.
• Given the polyclonal nature, this antibody may detect multiple epitopes on Cyclophilin 40, potentially enhancing signal in denaturing and native conditions.
• Consider testing in other applications such as immunohistochemistry or immunoprecipitation, though not validated here, by incorporating appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutes with integrated access to IVD raw materials, supporting projects from concept to clinic. With a focus on rare and validated targets like Cyclophilin 40, we offer bulk ancillary reagents, optimized monoclonal and polyclonal antibodies, and validated antibody pairs to accelerate your protein folding, cancer, or virology research. Partner with us for reliable, high-quality raw-material solutions.