Anti-Cyclophilin 40 Monoclonal Antibody for WB - Q08752

Core Product Specifications and Parameters

Biological Background: Cyclophilin 40 Function and Localization

• Cyclophilin 40, also known as PPID, CYP-40, or CYPD, is a 40 kDa peptidyl-prolyl cis-trans isomerase (PPIase) that catalyzes cis-trans isomerization of proline imidic peptide bonds, assisting protein folding (PubMed:11350175, PubMed:20676357). Related references: PMID:11350175 PMID:20676357
• It functions as a co-chaperone within HSP90 heterocomplexes, preferentially associating with unligated steroid receptors such as estrogen receptors, and may mediate cytoplasmic dynein-dependent nuclear translocation of the receptor.
• The protein is widely expressed and localized to both the cytoplasm and nucleus, including the nucleolus and nucleoplasm, consistent with its roles in protein folding and chaperone-mediated transport.
• Cyclophilin 40 participates in apoptosis regulation, promoting cell viability in certain lymphomas, and is implicated in UV radiation-induced apoptotic signaling.
• It is involved in host-virus interactions, potentially facilitating hepatitis C virus (HCV) replication and release.
• Post-translational modifications include acetylation and phosphorylation, and the protein contains TPR repeats that mediate protein-protein interactions.
• Keywords: Chaperone, isomerase, rotamase, nucleus, cytoplasm, TPR repeat, acetylation, phosphoprotein, transport, apoptosis.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide spanning amino acids 271–370 of human Cyclophilin 40. This C-terminal region may provide specific recognition of Cyclophilin 40.
• Predicted molecular weight is approximately 41 kDa; verify in your experimental system, as post-translational modifications may affect migration.
• For Western blot (WB), consider testing a range of dilutions on lysates from human, mouse, or rat cells, as cross-reactivity is reported.
• For IHC-P, ensure proper antigen retrieval and validate with appropriate positive and negative controls.
• ELISA applications may require optimization of coating antigen and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive IVD raw-material sourcing partner, providing diagnostic manufacturers and research institutes with one-stop access to validated antibodies, ancillary reagents, and technical services. Our portfolio includes optimized monoclonal antibodies like this anti-Cyclophilin 40 clone, ideal for chaperone biology and apoptosis research. We also support bulk procurement and rare target sourcing to streamline your assay development from concept to clinic.