Anti-Cyclin K Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - O75909

Core Product Specifications and Parameters

Biological Background: Cyclin-K Function and Localization

• Cyclin-K (CCNK) is a member of the cyclin family, functioning as a regulatory subunit of cyclin-dependent kinases (CDKs) that mediates target kinase activation.
• It plays a critical role in transcriptional regulation by controlling the phosphorylation of the C-terminal domain (CTD) of the large subunit of RNA polymerase II (POLR2A).
• The protein is localized to the nucleus, consistent with its role in transcription and cell cycle control.
• Cyclin-K is widely expressed across tissues, with the highest expression levels observed in the testis, suggesting potential tissue-specific functions.
• Post-translational modifications include phosphorylation (as indicated by the keyword "Phosphoprotein"), which may regulate its activity and interactions.
• Alternative splicing generates multiple transcript variants, contributing to functional diversity.
• Mutations in CCNK are associated with disease variants, including intellectual disability (IDDHDF), underscoring its importance in neurological development.
• Additional keywords: Cell cycle, Cell division, Cyclin, Host-virus interaction, Mitosis, Nucleus, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the N-terminal region (amino acids 1–160) of human Cyclin K, suggesting the antibody may be suitable for detecting both full-length and N-terminal fragments; consider epitope accessibility in your specific assay.
• For western blot (WB), a band at approximately 64 kDa is expected; validate in nuclear fractions or whole-cell lysates of positive tissues like testis.
• For immunohistochemistry (IHC-P), optimize antigen retrieval and validate in tissues known to express Cyclin K, such as testis; include controls to assess specificity.
• ELISA suitability is indicated; consider using the recombinant protein as a standard to evaluate antibody binding performance.
• Cross-reactivity with mouse and rat is reported, but validation in the relevant sample system is recommended before quantitative studies.
• As a polyclonal antibody, lot-to-lot variability is possible; always validate new batches using the intended applications.

CamelBio: Your One-Stop Sourcing Bridge

• CamelBio empowers diagnostic manufacturers and research labs with a comprehensive portfolio of IVD raw materials, including validated antibodies like this anti-Cyclin K rabbit polyclonal antibody. For cell cycle and transcription regulation studies, we also offer optimized monoclonal antibodies, calibrated antibody pairs, and bulk ancillary reagents to streamline your assay development. Our one-stop sourcing extends to rare-target raw materials, technical services, and consulting from concept to clinic, ensuring you have a reliable partner for every stage of your project.