Anti-Cyclin H Rabbit Polyclonal Antibody for WB, ELISA - P51946

Core Product Specifications and Parameters

Biological Background: Cyclin H Function and Localization

• Cyclin H is the regulatory subunit of the CDK-activating kinase (CAK) complex, which also contains the catalytic subunit CDK7 and the assembly factor MAT1. It binds and activates CDK7, enabling CAK to phosphorylate and activate CDK1, CDK2, CDK4, and CDK6, thereby regulating cell cycle progression. Related references: PMID:41100585
• In the context of the core-TFIIH basal transcription factor, Cyclin H-dependent CAK phosphorylates the C-terminal domain (CTD) of RNA polymerase II, facilitating promoter escape and transcript elongation. Related references: PMID:7533895, PMID:10024882
• Cyclin H is involved in both cell cycle control and RNA polymerase II-mediated transcription. Its expression and activity remain constant throughout the cell cycle.
• Subcellular localization: Cyclin H is predominantly nuclear.
• Keywords from UniProt indicate Cyclin H is a phosphoprotein and belongs to the cyclin family.
• Additional structural and functional insights are available from its 3D structures, as noted by the 3D-structure keyword.

Experimental Guidance and Technical Tips

• The immunogen is a full-length recombinant protein (amino acids 1–323), suggesting the antibody may recognize both native and denatured epitopes. For Western blot, consider standard denaturing conditions to expose linear epitopes.
• Validate the antibody in relevant human cell lines or tissues, as cross-reactivity is confirmed for human only. If working with other species, perform sequence alignment and pilot testing.
• For ELISA, optimize coating antigen concentration and use appropriate blocking buffers to minimize background.
• When probing phosphorylated forms of Cyclin H or its interactions with CDK7/MAT1, consider co‑immunoprecipitation or native gel electrophoresis, but note that such applications have not been validated with this product.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your integrated partner for IVD raw material procurement, providing validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and customized sourcing for rare targets. With this anti-Cyclin H antibody, we support research into cell cycle regulation and transcriptional control, enabling diagnostic developers and laboratories to accelerate their workflows. Our one-stop supply chain ensures consistent quality and reliable delivery for every stage from concept to clinic.