Anti-Cyclin H Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P51946

Core Product Specifications and Parameters

Biological Background: Cyclin-H Function and Localization

• Cyclin H (also known as MO15-associated protein, p34, or p37) is the regulatory subunit of the CDK-activating kinase (CAK) complex, a master regulator of cyclin-dependent kinase (CDK) activity. Related references: PMID:41100585
• It binds and activates CDK7, the catalytic subunit of CAK, which phosphorylates a critical threonine residue on CDK1, CDK2, CDK4, and CDK6, thereby promoting cell cycle progression. Related references: PMID:41100585
• When associated with the core-TFIIH basal transcription factor, CAK phosphorylates the C-terminal domain (CTD) of RNA polymerase II (POLR2A) on serine residues, facilitating promoter escape and transcript elongation. Related references: PMID:7533895, PMID:10024882
• Cyclin H is a member of the cyclin family, characterized by a conserved cyclin box domain, and its expression and activity remain constant throughout the cell cycle, unlike many other cyclins.
• The protein localizes predominantly to the nucleus, consistent with its roles in CDK activation and transcription regulation.
• Keywords from UniProt also highlight its involvement in cell cycle, transcription, and transcription regulation, and it is subject to phosphorylation.
• Cyclin H plays an essential role in both cell cycle control and RNA polymerase II-mediated transcription, linking these fundamental processes.

Experimental Guidance and Technical Tips

• The immunogen consists of a synthetic peptide from the C-terminal region (amino acids 224–323) of human Cyclin H. This region is likely to generate antibodies recognizing the full-length protein in denaturing and native conditions. Consider antigen retrieval methods for IHC-P to expose the epitope if it is masked.
• For Western blotting (WB), a band at approximately 38 kDa is anticipated in human and mouse samples. Always include appropriate positive and negative controls to confirm specificity.
• In immunofluorescence (IF/ICC), strong nuclear staining is expected based on Cyclin H’s subcellular localization. Co-staining with a nuclear marker is recommended to confirm localization.
• For ELISA applications, the antibody can be used as a capture or detection reagent, but optimal dilutions must be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw-material partner, offering validated antibody pairs, monoclonal and polyclonal antibodies, and bulk ancillary reagents to diagnostic manufacturers and research labs. This Cyclin H Rabbit mAb supports research and assay development in cell cycle regulation and transcription studies. For customized sourcing or rare target reagents, CamelBio provides end-to-end support from concept to clinic.