Anti-Cyclin H Polyclonal Antibody for WB, IP, ELISA - P51946

Core Product Specifications and Parameters

Biological Background: Cyclin H Function and Localization

• Cyclin H is the regulatory subunit of the CDK-activating kinase (CAK) complex, which acts as a master regulator of CDK activity by catalyzing the activating threonine phosphorylation of CDK1, CDK2, CDK4, and CDK6 (PubMed:41100585). Related references: PMID:41100585

• Within the TFIIH basal transcription factor complex, CAK phosphorylates serine residues of the C-terminal domain (CTD) of RNA polymerase II, enabling promoter escape and transcript elongation (PubMed:7533895, PubMed:10024882). Related references: PMID:7533895 PMID:10024882

• Cyclin H expression and activity remain constant throughout the cell cycle, underscoring its constitutive role in both cell cycle control and RNA polymerase II-driven transcription (PubMed:7533895, PubMed:10024882). Related references: PMID:7533895 PMID:10024882

• Subcellular localization is primarily nuclear, consistent with its functions in cell cycle regulation and transcription.

• Cyclin H is a member of the cyclin family and exists as a phosphoprotein, with phosphorylation likely modulating its interactions or stability.

Experimental Guidance and Technical Tips

• The immunogen spans nearly the full-length human Cyclin H protein (aa 1-323); the resulting polyclonal antibody may recognize native, denatured, and possibly post-translationally modified forms.
• For Western blot, consider enriching nuclear fractions to enhance signal given the nuclear localization of Cyclin H.
• In immunoprecipitation, titrate the antibody to optimize capture efficiency and minimize co-precipitation of associated proteins.
• For ELISA, the antibody may be used for direct or sandwich formats; validate with recombinant Cyclin H or endogenous protein in human cell lysates.
• The 38 kDa band may appear alongside minor bands due to polyclonal reactivity; confirm specificity using appropriate controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a one-stop sourcing solution for IVD raw materials, from concept to clinic. This anti-Cyclin H antibody, targeting a key regulator of cell cycle and transcription, exemplifies our commitment to supplying validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents. Reach out for technical support, custom development, or scalable procurement of rare-target raw materials.