Anti-Cyclin H Monoclonal Antibody for WB, ELISA - P51946

Core Product Specifications and Parameters

Biological Background: Cyclin H Function and Localization

• Cyclin H is the regulatory subunit of the CDK-activating kinase (CAK) complex, which includes CDK7 and MAT1. It binds and activates the cyclin-dependent kinase CDK7. Related references: PMID:41100585
• The CAK complex is essential for cell cycle progression by phosphorylating and activating CDK1, CDK2, CDK4, and CDK6. Related references: PMID:41100585
• In addition to its role in cell cycle regulation, the CAK complex is part of the core-TFIIH basal transcription factor, where it phosphorylates the C-terminal domain of RNA polymerase II to facilitate transcription initiation and elongation. Related references: PMID:7533895, PMID:10024882
• Cyclin H expression and activity remain constant throughout the cell cycle.
• Cyclin H localizes to the nucleus, consistent with its roles in CDK activation and transcriptional regulation.
• The protein is also known as MO15-associated protein, p34, and p37.
• Keywords associated with Cyclin H include cell cycle, cyclin, transcription, transcription regulation, and phosphoprotein.

Experimental Guidance and Technical Tips

• The immunogen was designed using a synthetic peptide from the N-terminal region (aa 1-100) of human Cyclin H; assess sequence homology before applying to non-human/non-mouse species for optimal reactivity.
• For Western blotting, anticipate a band near 38 kDa; use appropriate positive controls (e.g., HeLa, Jurkat lysates) and confirm specificity through siRNA knockdown or blocking peptide experiments.
• In ELISA applications, titrate both the capture/detection antibody and the antigen to determine optimal working concentrations, and confirm that the antibody recognizes the target in the desired native or recombinant form.
• Although validated to react with human and mouse, cross-reactivity with other species should be verified experimentally by sequence alignment and preliminary testing.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio delivers a comprehensive suite of IVD raw materials, from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents, supporting research and diagnostic development. For investigations into cell cycle dynamics and transcriptional control, this Cyclin H monoclonal antibody provides a reliable detection tool, and CamelBio can facilitate sourcing of additional rare targets or customized reagent panels to streamline your assay workflow from concept to clinical application.