Anti-Cyclin G1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P51959

Core Product Specifications and Parameters

Biological Background: Cyclin G1 Function and Localization

• Cyclin G1 (CCNG1) is a member of the cyclin family, which are key regulators of cell cycle progression and cell division.
• It is implicated in growth regulation and is associated with G2/M phase arrest in response to DNA damage, potentially acting downstream of p53.
• The protein localizes predominantly to the nucleus, consistent with its role in cell cycle control.
• High expression is observed in skeletal muscle, ovary, kidney, and colon, suggesting tissue-specific functions.
• Cyclin G1 undergoes alternative splicing, which may generate isoforms with distinct regulatory properties.
• As a cyclin, it participates in mitosis and is subject to proteomic identification as part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 200-295) of human Cyclin G1, which is conserved across human, mouse, and rat, supporting cross-reactivity.
• For Western blotting, a band at ~34 kDa is expected; optimize loading and transfer conditions for clear detection.
• In immunohistochemistry (IHC-P), consider heat-induced antigen retrieval and validate staining in positive control tissues such as ovary or colon.
• For ELISA, use the antibody as a capture or detection reagent and optimize coating conditions based on peptide/protein availability.
• Combining this antibody with p53-targeting reagents may offer insights into DNA damage response pathways.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a one-stop sourcing partner for IVD raw materials, offering validated antibodies and reagents for cell cycle and oncology research. For the Cyclin G1 polyclonal antibody, we support procurement of related cyclin antibodies, recombinant proteins, and ancillary reagents to streamline your diagnostic and research workflows.