Anti-Cyclin E2 Polyclonal Antibody for WB, IHC-P, ELISA - O96020

Core Product Specifications and Parameters

Biological Background: Cyclin E2 Function and Localization

• Cyclin E2 (CCNE2) is a G1/S-specific cyclin that controls cell cycle progression at the late G1 and early S phase.
• It forms a complex with CDK2, essential for the G1/S transition and initiation of DNA replication.
• The protein localizes to the nucleus, consistent with its role in regulating cell cycle checkpoints.
• Tissue expression: highest levels in adult testis, thymus, brain, and placenta; lower in spleen, colon, prostate, skeletal muscle, kidney; elevated in tumor-derived cells and lung. Related references: PMID:9858585, PMID:9840927, PMID:9840943
• Post-translational modifications include phosphorylation, indicating regulatory control by upstream signaling pathways.
• The gene undergoes alternative splicing, potentially generating isoforms with distinct functions.
• The reference proteome contains a protein of 46757 Da, with the 3D structure partially resolved.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide from the C-terminal region (aa 255-404), which may recognize full-length Cyclin E2 and potentially some isoforms.
• For WB, a band around 47 kDa is expected; validate in cell lines with known Cyclin E2 expression.
• For IHC-P, antigen retrieval and antibody titration are recommended; positive controls could include testis or tumor tissues where expression is high.
• For ELISA, peptide coating can be used to assess specificity. Always include appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated one-stop partner for IVD raw-material sourcing, supporting diagnostic developers, laboratories, and research institutes from concept to deployment. Our Cyclin E2 antibody portfolio, including validated polyclonal antibodies like this one, addresses cell cycle and oncology research demands. Beyond antibodies, we provide bulk ancillary reagents, rare target sourcing, and technical consultation to streamline your entire procurement process.