Anti-Cyclin E2 Polyclonal Antibody for WB, IF/ICC, ELISA - O96020

Core Product Specifications and Parameters

Biological Background: Cyclin E2 Function and Localization

• CCNE2 encodes G1/S-specific cyclin-E2, a regulatory subunit of cyclin-dependent kinases that governs cell cycle progression from G1 to S phase.
• Essential for the transition from late G1 to early S phase, facilitating DNA replication initiation.
• Localizes predominantly to the nucleus, consistent with its role in cell cycle control.
• Tissue expression varies: highest levels in adult testis, thymus, and brain; lower levels in placenta, spleen, colon, prostate, skeletal muscle, and kidney. Elevated expression also noted in lung (PubMed:9858585, PubMed:9840927, PubMed:9840943).
  Related references: PMID:9858585, PMID:9840927, PMID:9840943
• Consistently elevated in tumor-derived cells compared to non-transformed proliferating cells, suggesting involvement in oncogenesis (PubMed:9858585).
  Related references: PMID:9858585
• Subject to alternative splicing and phosphorylation, which may modulate its activity and stability.
• Keywords include cell cycle, cell division, cyclin, nucleus, phosphoprotein, and reference proteome; a 3D structure is available.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 255–404 of human Cyclin E2, a C-terminal region that may be conserved across isoforms; verify recognition of your isoform of interest.
• For Western blot, the predicted molecular weight is approximately 47 kDa; however, post-translational modifications may cause slight shifts.
• In immunofluorescence, expect nuclear staining; co-stain with a DNA marker to confirm localization.
• Validate antibody performance in your specific sample type, and include appropriate positive and negative controls (e.g., Cyclin E2-overexpressing cells, siRNA knockdown).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers integrated procurement solutions for IVD raw materials, covering antibodies, antigens, and ancillary reagents for cell cycle research. With this anti-Cyclin E2 polyclonal antibody, we support diagnostic developers and researchers in accessing validated polyclonal and monoclonal antibody pairs, bulk reagents, and rare target sourcing to accelerate assay development from concept to clinic.