Anti-Cyclin E1 Rabbit Polyclonal Antibody for WB and ELISA - P24864

Core Product Specifications and Parameters

Biological Background: Cyclin E1 Function and Localization

• Cyclin E1 is a G1/S-specific cyclin essential for cell cycle progression at the G1/S transition.
• It localizes to the nucleus, consistent with its role in regulating the cell cycle.
• The protein is subject to phosphorylation and ubiquitin conjugation, suggesting regulation by post-translational modifications.
• Cyclin E1 is highly expressed in testis and placenta, with low levels in bronchial epithelial cells.
• It belongs to the cyclin family, a group of proteins that control cell division.
• Alternative splicing results in multiple transcript variants.
• The protein has a molecular weight of approximately 47 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 300-400 of human Cyclin E1; the antibody may recognize the C-terminal region.
• For WB, consider using whole-cell lysates from proliferating cell lines (e.g., HeLa) as positive controls.
• Validate reactivity in mouse and rat samples due to documented cross-reactivity.
• Optimize antibody dilution and incubation conditions for the specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with a one-stop sourcing solution for IVD raw materials, from validated antibody pairs to bulk ancillary reagents. This anti-Cyclin E1 polyclonal antibody supports cell cycle and oncology research, complementing our extensive portfolio of optimized antibodies. Our supply chain can also accommodate rare target raw-material sourcing to accelerate your assay development.