Anti-Cyclin E1 Rabbit Monoclonal Antibody for WB & ELISA - P24864

Core Product Specifications and Parameters

Biological Background: Cyclin E1 Function and Localization

• Cyclin E1 (CCNE1) is a G1/S-specific cyclin essential for controlling the cell cycle transition from G1 to S phase.
• It functions as a regulatory subunit of cyclin-dependent kinase 2 (CDK2), promoting entry into S phase.
• The protein localizes to the nucleus, consistent with its role in cell cycle control.
• Tissue expression is highest in testis and placenta, with lower levels in bronchial epithelial cells.
• Keywords indicate alternative splicing, phosphorylation, and ubiquitin conjugation, suggesting regulatory post-translational modifications.
• Three-dimensional structural data is available, which may aid in antibody epitope characterization.

Experimental Guidance and Technical Tips

• The synthetic peptide immunogen (amino acids 300-400 of human Cyclin E1) targets a C-terminal region. This region may be well-exposed for antibody binding.
• For Western blotting, a band at approximately 47 kDa is expected. Consider validating with positive control lysates from testis or placenta, where Cyclin E1 is highly expressed.
• The antibody is suitable for ELISA; perform appropriate titrations and include negative controls to establish specificity.
• Cross-reactivity is reported for human, mouse, and rat; confirm reactivity in your specific model system before large-scale studies.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic developers and research laboratories with a comprehensive portfolio of IVD raw materials and services. This anti-Cyclin E1 antibody is an asset for cell cycle and oncology research applications. Beyond this product, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing assistance for rare targets to accelerate your assay development from concept to clinical application.