Anti-Cyclin E1 Monoclonal Antibody for WB, IHC-P, ELISA - P24864

Core Product Specifications and Parameters

Biological Background: Cyclin E1 Function and Localization

• Cyclin E1 (gene CCNE1) is a G1/S-specific cyclin that forms a complex with CDK2 to drive the G1/S transition, essential for cell cycle progression.
• It belongs to the highly conserved cyclin family, characterized by a cyclin box domain responsible for CDK binding and activation.
• The protein primarily localizes to the nucleus, where it regulates transcription and DNA replication initiation.
• Cyclin E1 is subject to multiple post-translational modifications, including phosphorylation and ubiquitination, which control its stability and timely degradation via the ubiquitin-proteasome pathway.
• Tissue expression is highest in testis and placenta, with lower levels detected in bronchial epithelial cells, indicating tissue-specific roles in proliferation.
• Alternative splicing generates isoforms that may have distinct functions or regulatory properties.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide spanning amino acids 300–400 of human Cyclin E1 (NP_001229.1); consider verifying epitope accessibility in your target sample.
• For Western blot (WB), use reducing conditions and load 20–30 µg of whole-cell lysate; a band near 47 kDa is expected. Validate detection with a known positive control (e.g., HeLa or MCF-7 cells).
• In immunohistochemistry (IHC-P), perform heat-induced antigen retrieval at pH 6 or 9, and optimize primary antibody dilution (suggested range 1:50–1:200). Include a tissue control with high Cyclin E1 expression.
• For ELISA, capture or detect recombinant Cyclin E1 or cell lysates; titrate the antibody to determine optimal coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your comprehensive partner for IVD raw-material sourcing, offering validated monoclonal antibodies like this Cyclin E1 antibody to support cell cycle and oncology research. Beyond single reagents, we supply optimized antibody pairs, bulk ancillary reagents, and facilitate custom sourcing for rare or specialized targets. Let CamelBio accelerate your assay development from concept to clinic with reliable, one-stop supply solutions.