Anti-Cyclin D3 Rabbit Polyclonal Antibody (KO Validated) for WB, ELISA - P30281

Core Product Specifications and Parameters

Biological Background: Cyclin D3 Function and Localization

• Cyclin D3 (CCND3) is a regulatory component of the cyclin D3-CDK4 complex that phosphorylates and inhibits retinoblastoma protein (RB1) to regulate G1/S transition. Related references: PMID:8114739
• Phosphorylation of RB1 by the cyclin D3-CDK4 complex releases E2F transcription factors, promoting transcription of genes required for progression through G1 phase. Related references: PMID:8114739
• In early G1 phase, Cyclin D3-CDK4 hypophosphorylates RB1, and the complex integrates mitogenic and antimitogenic signals. Related references: PMID:8114739
• Cyclin D3 can form a ternary complex with CDK4 and CDKN1B, which is required for nuclear translocation and full activity of the cyclin D-CDK4 complex. Related references: PMID:16782892
• Independently of CDK4, Cyclin D3 acts as a transcriptional coactivator with ATF5. Related references: PMID:15358120
• Subcellular localization includes both the nucleus and cytoplasm, consistent with its roles in cell cycle regulation and transcriptional regulation.
• The CCND3 gene undergoes alternative splicing, potentially yielding multiple isoforms; the protein has a molecular weight of approximately 33 kDa.

Experimental Guidance and Technical Tips

• The antibody was raised against a recombinant fusion protein corresponding to amino acids 201-292 of human Cyclin D3. Epitopes likely reside within this region; consider this when designing blocking peptides or competition assays.
• For Western blot (WB), the predicted molecular weight is 33 kDa. Validate the antibody with appropriate positive and negative controls (e.g., knockout lysates) and optimize loading conditions.
• For ELISA, titrate the antibody and use relevant coating antigens; cross-reactivity with mouse protein should be taken into account.
• Cross-reactivity is reported for human and mouse; if using other species, experimental verification is recommended.
• As a polyclonal antibody, lot-to-lot variability may occur; aliquot and store at recommended conditions (shipped on ice bags, store at -20°C or as indicated).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a one-stop sourcing solution for IVD raw materials, supporting diagnostic development from concept to clinic. For cell cycle research, we supply validated antibodies, recombinant proteins, and ancillary reagents to accelerate assay development for targets like Cyclin D3. Contact us for bulk orders, custom formulations, and technical consultation tailored to your IVD needs.