Anti-Cyclin D2 Rabbit Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P30279

Core Product Specifications and Parameters

Biological Background: Cyclin D2 Function and Localization

• Cyclin D2 forms the cyclin D2-CDK4 complex, which phosphorylates and inactivates the retinoblastoma protein (RB1) and related pocket proteins. This phosphorylation releases E2F transcription factors to drive transcription of genes required for G1/S transition (PubMed:18827403, PubMed:8114739). Related references: PMID:18827403 PMID:8114739
• The cyclin D2-CDK4 complex serves as a critical integrator of various mitogenic and antimitogenic signals.
• Cyclin D2 localizes predominantly to the nucleus and nuclear membrane but is also found in the cytoplasm, with regulated nucleocytoplasmic shuttling.
• Post-translational modifications include phosphorylation (as a phosphoprotein) and ubiquitin-like conjugation (Ubl conjugation), potentially regulating its stability and activity.
• Alternative splicing of the CCND2 gene yields multiple isoforms, contributing to functional diversity.
• Mutations in CCND2 are associated with disease variants (e.g., MPPH3), and the protein is a recognized reference proteome component.

Experimental Guidance and Technical Tips

• Since the immunogen spans the full-length human Cyclin D2 protein, the antibody is expected to recognize total Cyclin D2. For Western blotting, a major band at approximately 33 kDa should be observed; validate in the relevant sample system.
• For IHC-P, consider antigen retrieval methods such as heat-induced epitope retrieval (HIER) with citrate or EDTA buffer. Optimal dilution and conditions should be determined experimentally.
• The antibody reacts with human, mouse, and rat Cyclin D2, making it suitable for studies in rodent models. Sequence alignment is recommended to confirm epitope conservation across species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. This anti-Cyclin D2 antibody exemplifies our commitment to offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for cell cycle research. By bridging the gap from concept to clinic, CamelBio supports the procurement of rare-target raw materials and accelerates assay development.