Anti-Cyclin D2 Rabbit Polyclonal Antibody for WB and ELISA - P30279

Core Product Specifications and Parameters

Biological Background: Cyclin D2 Function and Localization

• Cyclin D2 is a G1/S-specific cyclin that assembles with CDK4 to form an active kinase complex essential for cell cycle progression. Related references: PMID:18827403, PMID:8114739
• The cyclin D2-CDK4 complex phosphorylates retinoblastoma protein (RB1) and related family members, leading to the release of E2F transcription factors and transcription of genes required for G1 phase transition. Related references: PMID:18827403, PMID:8114739
• This complex serves as a central integrator of mitogenic and antimitogenic signals, linking extracellular cues to cell cycle entry. Related references: PMID:18827403, PMID:8114739
• Cyclin D2 localizes primarily to the nucleus but is also found in the cytoplasm and at the nuclear membrane, reflecting dynamic regulation.
• Alternative splicing generates multiple isoforms, and the protein is subject to post-translational modifications including phosphorylation and ubiquitin-like conjugation.
• Mutations in the CCND2 gene are associated with disease phenotypes (keyword: Disease variant).

Experimental Guidance and Technical Tips

• The immunogen encompasses the full-length N‑terminal region (aa 1‑289), which is conserved across human, mouse, and rat, explaining the reported multispecies cross‑reactivity.
• For Western blot, consider using whole‑cell lysates from proliferating cell lines (e.g., HeLa, 293T) where Cyclin D2 is expressed; optimization of lysis conditions may be needed to preserve phosphorylation status.
• For ELISA, validate the antibody against recombinant Cyclin D2 protein and consider using a matched antibody pair in a sandwich format if quantitative detection is required.
• Always include appropriate positive and negative controls (e.g., lysates from known Cyclin D2‑positive and knockout cells) and titrate the primary antibody to determine the optimal working concentration for your specific assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw‑material procurement with a comprehensive portfolio that includes validated antibodies, matched pairs, recombinant proteins, and ancillary reagents. For cell cycle and oncology research, we offer products such as this Cyclin D2 polyclonal antibody, backed by our capability to source rare‑target raw materials and supply bulk quantities. Partner with CamelBio to accelerate your assay development from concept to clinic.