Anti-Cyclin D1 Rabbit Polyclonal Antibody for WB - P24385

Core Product Specifications and Parameters

Biological Background: Cyclin D1 Function and Localization

• Cyclin D1 (CCND1), also known as BCL-1 or PRAD1 oncogene, is a G1/S-specific cyclin that plays a central role in cell cycle progression.
• It serves as the regulatory subunit of the cyclin D1-CDK4 complex, which phosphorylates and inhibits retinoblastoma (RB) proteins, releasing E2F transcription factors to drive G1/S transition. Related references: PMID:1827756 PMID:1833066 PMID:19412162
• The cyclin D1-CDK4 complex integrates various mitogenic and antimitogenic signals, committing the cell to division. Related references: PMID:1827756 PMID:1833066 PMID:19412162
• Cyclin D1 phosphorylates SMAD3 in a cell-cycle-dependent manner, repressing its transcriptional activity and providing a link between cell cycle and TGF-β signaling. Related references: PMID:15241418
• It is a component of the cyclin D1/CDK4/CDKN1B (p27) ternary complex, which is required for nuclear translocation and activation of the kinase. Related references: PMID:9106657
• Cyclin D1 exhibits cell-cycle-independent transcriptional corepressor activity with INSM1 on promoters such as NEUROD1 and INS, expanding its role beyond cell division. Related references: PMID:16569215 PMID:18417529
• Subcellular localization is primarily nuclear, with additional presence in the cytoplasm and at the nuclear membrane.
• The protein is frequently dysregulated in cancer as a proto-oncogene and is subject to chromosomal rearrangements, phosphorylation, and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic peptide corresponding to residues 200–295 of human Cyclin D1 (NP_444284.1). Consider this region when evaluating isoform or variant detection.
• For Western blot, a band around 34 kDa is expected. Validate in your specific sample system and optimize loading controls.
• The antibody is suitable for multiple applications including IF/ICC, IP, and ELISA. Titrate and validate for each assay format individually.
• Cross-reactivity with mouse and rat is reported; however, confirm reactivity in your experimental models due to potential sequence variations.
• For immunoprecipitation, ensure appropriate negative controls and consider the end use (e.g., kinase activity, mass spectrometry) to maintain complex integrity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides this anti-Cyclin D1 rabbit polyclonal antibody as part of a comprehensive IVD raw-material portfolio for cell cycle and oncology research. Our supply chain covers validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents to support diagnostic kit development. For rare target sourcing or custom technical consulting, our one-stop platform bridges the gap from concept to clinic.