Anti-Cyclin D1 Rabbit Polyclonal Antibody for WB, IP, IF/ICC, IHC-P, ELISA - P24385

Core Product Specifications and Parameters

Biological Background: Cyclin D1 Function and Localization

• Cyclin D1 (also known as BCL1, PRAD1) is a G1/S-specific cyclin that acts as a regulatory subunit of CDK4/CDK6, essential for cell cycle progression.
• The cyclin D1-CDK4 complex phosphorylates retinoblastoma (RB1) and related proteins, leading to release of E2F transcription factors and transcription of genes required for G1/S transition. Related references: PMID:1827756, PMID:1833066, PMID:19412162
• Cyclin D1 assembles into the ternary complex cyclin D1/CDK4/CDKN1B, which is necessary for its nuclear translocation and kinase activity. Related references: PMID:9106657
• In addition to its role in cell cycle, Cyclin D1 exhibits transcriptional corepressor activity with INSM1 on promoters such as NEUROD1 and INS, functioning independently of the cell cycle. Related references: PMID:16569215, PMID:18417529
• Cyclin D1 is also a substrate for SMAD3, leading to SMAD3 phosphorylation and repression of its transcriptional activity in a cell-cycle-dependent manner. Related references: PMID:15241418
• Subcellularly, Cyclin D1 localizes to the nucleus, cytoplasm, and nucleus membrane, reflecting its dynamic roles in signaling and transcription.
• CCND1 is a proto-oncogene frequently targeted by chromosomal rearrangements in B-cell malignancies, particularly mantle cell lymphoma, and is a key integration point for mitogenic and antimitogenic signals.
• The protein undergoes post-translational modifications including phosphorylation and ubiquitin conjugation, which regulate its stability and activity.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 200-295, a region distinct from the cyclin box; consider this when designing epitope mapping or competitive assays.
• For Western blot, the predicted molecular weight is ~34 kDa; however, Cyclin D1 may appear as multiple bands due to phosphorylation or isoform variations.
• In immunoprecipitation, use appropriate lysis buffers to preserve protein-protein interactions involving CDK4/CDKN1B.
• For immunohistochemistry, antigen retrieval methods may be required depending on tissue fixation; validate in the relevant sample system.
• Cross-reactivity with mouse and rat is expected; confirm in your specific model.
• ELISA applications may require optimization of coating and detection conditions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents. For investigators studying cell cycle regulation and oncogenic pathways, CamelBio offers one-stop access to Cyclin D1 antibodies and associated reagents, supporting your project from initial research to clinical application. Explore our capabilities in rare target sourcing and technical consulting to accelerate your diagnostic development.