Anti-Cyclin C Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P24863

Core Product Specifications and Parameters

Biological Background: Cyclin C Function and Localization

• Cyclin C is an essential subunit of the Mediator complex, a coactivator that bridges gene-specific transcription factors with the basal RNA polymerase II transcription machinery.
• It binds and activates cyclin-dependent kinases CDK8 and CDK19, which phosphorylate the C-terminal domain (CTD) of the RNA polymerase II large subunit, potentially inhibiting transcription initiation complex formation.
• Mediator complex mediates regulated transcription of nearly all RNA polymerase II-dependent genes, conveying regulatory signals to the core promoter.
• Alternative splicing of CCNC generates multiple isoforms, contributing to functional diversity in transcription regulation.
• Localizes exclusively to the nucleus, consistent with its role as a transcriptional regulator.
• Highest expression in the pancreas; also abundant in heart, liver, skeletal muscle, and kidney. Low expression in brain.
• Keywords: Activator, Repressor, Cyclin, Nucleus, Phosphoprotein, Proteomics identification, Reference proteome, Transcription, Transcription regulation.

Experimental Guidance and Technical Tips

• The immunogen (full-length recombinant Cyclin C, amino acids 1-283) ensures broad epitope coverage; the antibody is suitable for detecting total Cyclin C in western blotting.
• For WB, expect a band at approximately 33 kDa; use a nuclear loading control (e.g., Lamin B1) for normalization.
• In IHC-P, consider optimizing antigen retrieval (citrate buffer pH 6.0 or Tris-EDTA pH 9.0) and validating on positive control tissues (e.g., pancreas or liver sections).
• The antibody cross-reacts with mouse and rat, making it useful for translational studies; however, sequence alignment is recommended to confirm epitope conservation.
• As a polyclonal antibody, lot-to-lot consistency should be assessed for quantitative assays; recombinant protein batches can serve as reference standards.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a reliable, single-source solution for IVD raw materials, from validated antibodies to bulk ancillary reagents. With expertise in transcription and cell cycle targets like Cyclin C, we streamline procurement of optimized antibody pairs, polyclonal and monoclonal antibodies, and rare-target raw materials to support your assay development from concept to clinic. Contact our team to discuss your customized sourcing needs.