Anti-Cyclin C Polyclonal Antibody for WB and ELISA - P24863

Core Product Specifications and Parameters

Biological Background: Cyclin C Function and Localization

• Cyclin C, encoded by the CCNC gene, is the human homolog of yeast SRB11 and a member of the cyclin family, functioning as a regulatory subunit of cyclin-dependent kinases CDK8 and CDK19.
• It is a core component of the Mediator complex, a large coactivator essential for the regulation of nearly all RNA polymerase II-dependent gene transcription, bridging gene-specific activators and the basal transcription machinery.
• Cyclin C binds and activates CDK8/CDK19, which phosphorylate the C-terminal domain (CTD) of the RNA polymerase II largest subunit; this phosphorylation can inhibit the formation of a functional transcription initiation complex.
• The protein localizes to the nucleus, consistent with its role in transcriptional control.
• Cyclin C is ubiquitously expressed, with highest levels in the pancreas, high levels in heart, liver, skeletal muscle, and kidney, and low levels in the brain.
• Post‑translational modifications and alternative splicing are reported, and the protein is classified as both a transcription activator and repressor, reflecting its context‑dependent regulatory functions.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 1‑283 of human Cyclin C; the polyclonal nature ensures recognition of multiple epitopes, but cross‑reactivity in untested species should be validated.
• For Western blot, the calculated molecular weight is approximately 33 kDa; use appropriate lysis buffers and load controls, and confirm band specificity in target tissues based on the manufacturer’s validation.
• ELISA applications can be developed using this antibody as a capture or detection reagent; optimize coating and blocking conditions for your assay format.
• Cross‑reactivity with mouse and rat makes this antibody suitable for studies in common preclinical models; verify expression in your specific cell or tissue samples.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research laboratories with a comprehensive portfolio of IVD raw materials, including validated polyclonal antibodies like this anti‑Cyclin C reagent for transcription‑focused assays. We offer one‑stop access to optimized antibody pairs, ancillary reagents, and rare‑target sourcing to accelerate your development from concept to clinic. Let our supply capabilities support your transcription regulation research and immunoassay projects.