Anti-Cyclin B2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, ELISA - O95067

Core Product Specifications and Parameters

Biological Background: Cyclin B2 Function and Localization

• Cyclin B2 (UniProt: O95067), also referred to as HsT17299, is a B-type cyclin encoded by the CCNB2 gene in humans and is a member of the cyclin family of cell cycle regulators.
• It is essential for the control of the cell cycle at the G2/M transition, forming complexes with cyclin-dependent kinase 1 (CDK1) to drive mitotic entry.
• The protein is classified under keywords including Cell cycle, Cell division, Cyclin, and Mitosis, reflecting its role in promoting cell proliferation.
• Cyclin B2 is annotated as a phosphoprotein, indicating that its activity and degradation are regulated by phosphorylation events during the cell cycle.
• The Swiss-Prot record for O95067 does not detail subcellular localization, tissue specificity, or isoform diversity; further data may be available in specialized databases.
• No antimicrobial or tissue-specific functions are described in the current record.
• Its calculated molecular mass is 45,282 Da, consistent with the ~45 kDa band detected by this antibody in Western blotting.

Experimental Guidance and Technical Tips

• The antibody was generated against a synthetic peptide spanning amino acids 1–100 of human cyclin B2. This N-terminal region is distinct from cyclin B1; however, users should verify specificity by sequence alignment if cross-reactivity is a concern.
• For Western blot, a band around 45 kDa is expected. Include a positive control such as lysates from cells synchronized at the G2/M phase, where cyclin B2 expression peaks.
• In immunohistochemistry (IHC-P), antigen retrieval methods (e.g., heat-induced epitope retrieval with citrate buffer pH 6.0 or EDTA pH 9.0) may be optimized by the end user.
• For immunofluorescence (IF/ICC), fixation with 4% paraformaldehyde and permeabilization with 0.1–0.5% Triton X‑100 are typical starting points; staining patterns may depend on cell cycle stage.
• ELISA performance should be validated using recombinant cyclin B2 or cell lysates; titration of both capture and detection reagents is recommended.
• The antibody’s documented cross-reactivity with mouse and rat implies potential utility in these species, but conservation of the immunogen sequence should be confirmed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers and research teams with a comprehensive range of IVD raw materials, including validated antibody pairs whether monoclonal or polyclonal, optimized antibodies, and bulk ancillary reagents. For cell cycle and mitosis studies, we offer reliable sourcing of cyclin B2 detection tools and can assist in procuring rare-target raw materials. Let our procurement network streamline your development from concept to clinic.