Anti-Cyclin B1 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P14635

Core Product Specifications and Parameters

Biological Background: Cyclin B1 Function and Localization

• Cyclin B1 (CCNB1) is a member of the cyclin family, encoded by the CCNB1 gene, which undergoes alternative splicing to produce multiple isoforms.
• It is a key cell cycle regulator, essential for the G2/M phase transition. Cyclin B1 binds and activates cyclin-dependent kinase CDK1/CDC2, forming the M-phase promoting factor (MPF). Related references: PMID:41100585
• The Cyclin B1-CDK1 complex phosphorylates substrates critical for mitotic entry, including those involved in nuclear envelope breakdown and chromosome condensation. Related references: PMID:27030811 PMID:17495531 PMID:17495533
• Subcellular localization: Cyclin B1 shuttles between the cytoplasm and nucleus, and localizes to the centrosome at the microtubule organizing center during mitosis.
• Post-translational modifications: Cyclin B1 is a phosphoprotein and undergoes acetylation and ubiquitination, which regulate its stability and degradation, ensuring timely mitotic exit.
• UniProt categorizes Cyclin B1 under keywords including cell cycle, cell division, cyclin, mitosis, and phosphoprotein, with available 3D structural data.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the C-terminal region (amino acids 334–433) of human Cyclin B1. This peptide may ensure recognition of the full-length protein and potentially certain isoforms.
• For Western blot (WB), the predicted molecular weight is ~48 kDa, though post-translational modifications may cause shifts; validate in relevant sample systems.
• In immunofluorescence/immunocytochemistry (IF/ICC), consider fixation and permeabilization conditions to preserve Cyclin B1 localization, particularly its dynamic shuttling during the cell cycle.
• For ELISA, this polyclonal antibody can be used as either capture or detection antibody, but optimal dilutions should be empirically determined.
• Cross-reactivity with mouse and rat Cyclin B1 has been reported; confirm performance in your specific species/tissue context.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for IVD raw-material sourcing, supporting developers from concept to clinic. This Cyclin B1 antibody, targeting a pivotal cell cycle regulator, is relevant for oncology and cell proliferation research. Beyond polyclonal antibodies, CamelBio offers validated pairs, optimized monoclonals, bulk ancillary reagents, and rare-target sourcing to streamline your workflow.