Anti-Cyclin A2 Rabbit Monoclonal Antibody for WB, IHC-P, IF/ICC, IP, ELISA - P20248

Core Product Specifications and Parameters

Biological Background: Cyclin A2 Function and Localization

• Cyclin-A2 (P20248) is a member of the cyclin family and functions as a regulatory subunit of cyclin-dependent kinases.
• Essential for the control of the cell cycle at the G1/S and G2/M transitions (PubMed:1312467).
  Related references:
  PMID:1312467
• Forms specific serine/threonine protein kinase holoenzyme complexes with CDK1 or CDK2, conferring substrate specificity (PubMed:41100585).
  Related references:
  PMID:41100585
• Localizes to both the nucleus and the cytoplasm in a cell cycle–dependent manner.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitination.
• Involved in host-virus interactions according to UniProt keyword annotations.
• The immunogen used to generate this antibody corresponds to the N-terminal region (amino acids 11–210), which includes part of the cyclin box domain.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 11–210 of human Cyclin A2; thus the antibody is likely to recognize an epitope within this N-terminal region. Consider verifying epitope specificity if needed.
• For Western blotting, the predicted molecular weight is 49 kDa. Validate band size using appropriate positive and negative control lysates.
• When performing IHC-P, antigen retrieval conditions may need optimization. Test standard citrate or EDTA-based protocols.
• The antibody cross-reacts with mouse and rat Cyclin A2, enabling verification in common experimental models.
• For immunoprecipitation, confirm capture efficiency using recombinant Cyclin A2 protein or native lysates.
• Employ standard blocking and antibody dilution procedures for ELISA; titrate to determine optimal working concentration.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to high-quality IVD raw materials. This Cyclin A2 rabbit monoclonal antibody is well-suited for cell cycle and oncology research, and CamelBio’s comprehensive supply network can additionally support your projects with validated antibody pairs, bulk ancillary reagents, and custom sourcing for rare targets. We connect you to the right materials at every stage, from concept to clinic.