Anti-Cyclin A2 Polyclonal Antibody for WB, IHC-P, IF/ICC, ELISA - P20248

Core Product Specifications and Parameters

Biological Background: Cyclin-A2 Function and Localization

• Cyclin-A2 (CCNA2), also known as Cyclin A, is a key regulator of the cell cycle, essential for the G1/S and G2/M transitions. Related references: PMID:1312467
• It functions by forming active kinase holoenzyme complexes with cyclin-dependent kinases CDK1 and CDK2, where the cyclin subunit dictates substrate specificity and differentially regulates CDK activity throughout the cell cycle. Related references: PMID:41100585
• Cyclin-A2 is the predominant A-type cyclin in somatic cells, with the CCNA2 gene located on chromosome 4q27 and a calculated molecular weight of approximately 49 kDa.
• Subcellular localization includes both the nucleus and cytoplasm, with nuclear accumulation peaking during S and G2 phases of the cell cycle.
• Post-translational modifications such as phosphorylation, acetylation, and ubiquitination regulate its stability, activity, and degradation.
• As a member of the cyclin family, Cyclin-A2 plays a critical role in cell division, mitosis, and host-virus interactions, and it serves as a reference proteome marker.

Experimental Guidance and Technical Tips

• The immunogen used is a synthetic peptide corresponding to the C-terminal region (amino acids 350-432) of human Cyclin A2 (NP_001228.1), which may produce antibodies recognizing the full-length protein and potentially N‑terminal isoforms.
• For Western blotting, the expected band is ~49 kDa; consider using appropriate reducing conditions and validate with positive and negative control lysates.
• In IHC-P applications, antigen retrieval protocols (e.g., heat-induced epitope retrieval) should be optimized for the target tissue, and include isotype controls.
• For IF/ICC, fixation and permeabilization conditions must be validated; the antibody may detect Cyclin A2 in both cytoplasmic and nuclear compartments.
• Since it is a polyclonal antibody, lot-to-lot consistency should be verified in the intended assay, and cross-reactivity with other cyclin family members cannot be excluded without additional testing.
• All recommended applications and species cross-reactivity (human, mouse, rat) are based on supplier specifications; independent validation in the user’s experimental system is essential.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting—covering every stage from concept to clinic. In cell cycle and oncology research, we offer validated Cyclin A2 antibodies, as well as bulk ancillary reagents and customized raw-material sourcing for rare targets. Partner with us to streamline your assay development and secure a reliable supply chain.