Anti-Cyclin A1/A2 Rabbit Monoclonal Antibody for WB, IP, ELISA - P20248 / P78396

Core Product Specifications and Parameters

Biological Background: Cyclin A2 Function and Localization

• Cyclin A2 is essential for cell cycle progression, controlling the G1/S and G2/M transitions. Related references: PMID:1312467
• It functions by forming complexes with cyclin-dependent kinases CDK1 or CDK2, conferring substrate specificity and driving cell cycle phases. Related references: PMID:41100585
• Cyclin A2 localizes to both the nucleus and cytoplasm.
• The protein undergoes various post-translational modifications, including acetylation, phosphorylation, and ubiquitination.
• It is a member of the cyclin protein family and serves as a reference proteome in multiple identification studies.

Experimental Guidance and Technical Tips

• The immunogen spans the C-terminal region (aa 250-465) of human Cyclin A1/A2, enabling detection of both isoforms and likely cross-reactivity with mouse due to sequence conservation.
• For Western blot (WB), expect a band around 49-52 kDa in human cell lysates; consider using appropriate loading controls.
• In immunoprecipitation (IP), use standard protocols with protein A/G beads, as the antibody is rabbit monoclonal.
• ELISA performance can be assessed using recombinant Cyclin A proteins; validate with relevant sample matrices.
• Always optimize antibody dilution and incubation conditions for your specific experimental setup.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio partners with diagnostic manufacturers and research labs to supply high-quality IVD raw materials, such as this Cyclin A1/A2 antibody for cell cycle studies. We offer a comprehensive portfolio including validated antibody pairs, bulk ancillary reagents, and tailored sourcing for rare targets. Contact us to streamline your procurement and accelerate development.