Anti-Cyclin A1 Polyclonal Antibody for WB, ELISA - P78396

Core Product Specifications and Parameters

Biological Background: Cyclin A1 Function and Localization

• Cyclin A1, encoded by the CCNA1 gene (GeneID: 8900), is a member of the cyclin family with a predicted molecular weight of approximately 52 kDa (UniProt P78396).
• It functions in cell cycle regulation, potentially controlling both G1/S and G2/M phase transitions.
• Cyclin A1 is predominantly expressed in the testis, with very low levels in the brain, and is also detectable in myeloid leukemia cell lines, reflecting its primary role in germline meiotic cell cycle and additional mitotic functions in some somatic cells.
• The protein localizes to the nucleus, consistent with its involvement in cell cycle control.
• Post‑translational modifications, including ubiquitin conjugation (Ubl conjugation) and alternative splicing, may regulate its activity and stability.
• Keywords from UniProt highlight its role in cell cycle, cell division, mitosis, and its nuclear localization, underscoring its importance in proliferative processes.

Experimental Guidance and Technical Tips

• The immunogen for this antibody is a synthetic peptide spanning residues 350‑450 of human CCNA1 (NP_001104515.1); consider using the recombinant protein or peptide in ELISA validation.
• For Western blot analysis, testis or myeloid leukemia cell lysates (e.g., K562) are potential samples due to high Cyclin A1 expression; verify the ~52 kDa band with appropriate controls.
• Cross‑reactivity with mouse and rat is indicated; if using rodent models, validate antibody specificity in the relevant tissue or cell line.
• ELISA applications may require optimization of coating antigen and antibody dilution; pairing with a confirmed detection antibody could improve assay performance.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with reliable access to IVD raw materials, from validated antibodies and antigen pairs to ancillary reagents. This Cyclin A1 antibody supports cell cycle and oncology research, and is part of our curated portfolio for meiosis and proliferation studies. For custom raw‑material sourcing or technical consultation, our team offers tailored supply solutions to help accelerate your assay development.