Anti-CYC1 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P08574

Core Product Specifications and Parameters

Biological Background: CYC1 Function and Localization

• CYC1 encodes cytochrome c1, a heme protein that is part of the mitochondrial respiratory chain complex III (ubiquinol-cytochrome c oxidoreductase). It is also known as Complex III subunit 4, UQCR4, and MC3DN6.
• Cytochrome c1 is a catalytic core subunit containing a c-type heme group that transfers electrons from the Rieske iron-sulfur protein to cytochrome c.
• The cytochrome b-c1 complex catalyzes the transfer of electrons from ubiquinol to cytochrome c, coupling this redox reaction to proton translocation across the mitochondrial inner membrane via the Q cycle.
• Located in the mitochondrial inner membrane, CYC1 is a single-pass transmembrane protein with a transit peptide for mitochondrial import.
• Cytochrome c1 is subject to phosphorylation, which may regulate its activity or assembly.
• Mutations in CYC1 are associated with primary mitochondrial disease, specifically mitochondrial complex III deficiency, nuclear type 6 (MC3DN6).
• The mature protein has a molecular weight of approximately 35 kDa (35422 Da from UniProt, consistent with observed 35 kDa on SDS-PAGE).

Experimental Guidance and Technical Tips

• The antibody is raised against a recombinant protein spanning amino acids 85-291 of human CYC1 (NP_001907.2). This immunogen covers the mature peptide after removal of the mitochondrial targeting sequence, and includes the heme-binding and transmembrane domains.
• For Western blotting, a predominant band at approximately 35 kDa is expected in human, mouse, and rat mitochondrial extracts. Validate loading controls and consider using protease inhibitors to prevent degradation.
• In immunohistochemistry (IHC-P), antigen retrieval may be required; test both heat-induced and enzymatic methods. For ELISA, direct coating of the recombinant immunogen or purified complex III may be considered as a positive control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio sources validated antibody pairs, optimized polyclonal and monoclonal antibodies, and bulk ancillary reagents for IVD assay development. For mitochondrial and metabolism research, we supply raw materials targeting key electron transport chain components such as CYC1, enabling seamless transition from concept to clinical application.