Anti-CYBRD1 Polyclonal Antibody for WB, ELISA - Q53TN4

Core Product Specifications and Parameters

Biological Background: CYBRD1 Function and Localization

• CYBRD1, also known as duodenal cytochrome b (DCYTB), is a member of the cytochrome b561 family, characterized by heme binding and multiple transmembrane domains.
• Acts as a plasma membrane ascorbate-dependent ferrireductase, converting extracellular Fe3+ to Fe2+ for dietary iron absorption in duodenal enterocytes. Related references: PMID:30272000
• Reduces extracellular monodehydro-L-ascorbate, potentially participating in ascorbate regeneration by erythrocytes. Related references: PMID:17068337
• May also function as a cupric transmembrane reductase and as a ferrireductase in airway epithelial cells.
• Localizes at the apical cell membrane of duodenal enterocytes and is present in erythrocyte membranes.
• Detected at the protein level in erythrocyte membranes and also expressed in respiratory epithelium.
• Post-translational modifications include phosphorylation; structural data are available (3D-structure).

Experimental Guidance and Technical Tips

• Given the immunogen spans amino acids 215-286, the antibody likely recognizes a conserved internal epitope, suitable for detecting both full-length and truncated forms under denaturing conditions in Western blotting.
• For Western blot, consider using a loading control and optimizing primary antibody concentration; the predicted band size is approximately 32 kDa.
• For ELISA, validate antibody specificity with the recombinant immunogen or native protein in the relevant sample matrix (e.g., human duodenal or erythrocyte lysates).
• Cross-reactivity has been demonstrated with human CYBRD1; reactivity with other species is not specified and should be verified if needed.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides a comprehensive one-stop sourcing platform for IVD raw materials, supporting diagnostic manufacturers and researchers in iron metabolism and ferrireductase studies. Our portfolio includes validated antibody pairs, optimized polyclonal antibodies like anti-CYBRD1, and bulk ancillary reagents, along with expert consulting to streamline development from concept to clinic. For rare or custom targets, our sourcing bridge connects you with reliable supply chains.