Anti-CYBA Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - P13498

Core Product Specifications and Parameters

Biological Background: CYBA (p22phox) Function and Localization

• CYBA encodes the p22phox protein, the light chain of the cytochrome b-245 complex (also known as cytochrome b(558)), which is an essential subunit of the phagocyte NADPH oxidase complex. Related references: PMID:15824103, PMID:17140397, PMID:38355798

• The NADPH oxidase complex transfers electrons from cytosolic NADPH to molecular oxygen, generating the superoxide anion (O2(-)), a reactive oxygen species crucial for host defense and cell signaling. Related references: PMID:38355798

• p22phox acts as a scaffold for the assembly of cytosolic regulatory subunits, and its C-terminal region is phosphorylated, allowing binding of NCF1/p47-phox to initiate complex activation. Related references: PMID:19948736

• CYBA also associates with NOX3 to form a constitutively active NADPH oxidase that generates superoxide in non-phagocytic cells. Related references: PMID:15824103, PMID:17140397

• The protein localizes to the cell membrane and phagosomal membrane upon activation.

• CYBA contains transmembrane helices and binds heme and iron cofactors essential for electron transfer.

• Mutations in CYBA are associated with chronic granulomatous disease (CGD), a primary immunodeficiency characterized by defective superoxide production.

• The protein is subject to post-translational modifications including phosphorylation and ubiquitination.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 126–195 of human CYBA, a region that is conserved across human, mouse, and rat, consistent with the observed cross-reactivity. For detection of endogenous CYBA, consider validating the antibody in relevant cell lines (e.g., neutrophils, macrophages) or tissues.
• For western blotting, a predicted molecular weight of 21 kDa is expected. However, post-translational modifications may cause slight shifts. Include appropriate positive and negative controls.
• In immunofluorescence applications, CYBA localizes to the plasma membrane, so membrane staining patterns should be observed. Permeabilization conditions must be optimized for the target cell type.
• ELISA protocols should be optimized with a suitable coating buffer and detection system, ensuring proper blocking to reduce nonspecific binding.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your trusted partner for IVD raw material procurement, offering a broad portfolio of high-quality antibodies and ancillary reagents for diagnostic development. Our anti-CYBA polyclonal antibody supports research into phagocyte oxidase function, oxidative stress pathways, and immune disorders. Beyond antibodies, we assist with custom sourcing of rare targets, bulk reagent supply, and technical consulting to streamline your project from concept to clinic.