Anti-CYB5R3 Polyclonal Antibody (KD Validated) for WB and ELISA - P00387

Core Product Specifications and Parameters

Biological Background: CYB5R3 (NADH-Cytochrome b5 Reductase 3) Function and Localization

• CYB5R3, also known as NADH-cytochrome b5 reductase 3 or diaphorase-1, is a flavoprotein belonging to the oxidoreductase family.
• It catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor.
• The CYB5R3 gene generates multiple isoforms through alternative promoter usage and alternative splicing.
• Subcellularly, it localizes to the endoplasmic reticulum membrane, mitochondrial outer membrane, and cytoplasm.
• Expression is noted at late stages of erythroid maturation, indicating a role in erythroid function.
• Post-translational modifications include acetylation, phosphorylation, and myristoylation.
• Functional involvement extends to cholesterol biosynthesis, lipid metabolism, and steroid metabolism.

Experimental Guidance and Technical Tips

• The immunogen covers amino acids 27-301 of human CYB5R3, encompassing a large portion of the protein, which may support broad reactivity.
• For Western blot, anticipate multiple bands corresponding to isoforms at approximately 31, 34, and 38 kDa.
• Validate antibody specificity in the relevant species (human, rat) and appropriate biological samples.
• When performing ELISA, optimize antigen coating and detection conditions for best results.
• Consider subcellular fractionation or marker controls when studying localization due to CYB5R3's presence in multiple organelles.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material sourcing partner, connecting diagnostic developers with validated antibody pairs, optimized polyclonal antibodies, and bulk ancillary reagents. For research on CYB5R3 and related metabolic pathways, we provide high-quality raw materials to accelerate your assay development. Explore our catalog for rare-target sourcing and custom antibody solutions tailored to your project needs.