Anti-CYB5R3 Polyclonal Antibody for WB, IF/ICC, ELISA - P00387

Core Product Specifications and Parameters

Biological Background: CYB5R3 Function and Localization

• NADH-cytochrome b5 reductase 3 (CYB5R3), also known as diaphorase-1, is a flavoprotein (FAD-containing) enzyme encoded by the CYB5R3 (DIA1) gene in humans.
• CYB5R3 catalyzes the reduction of two molecules of cytochrome b5 using NADH as an electron donor, playing a crucial role in electron transport and redox reactions.
• The enzyme is involved in lipid metabolism, including cholesterol biosynthesis and fatty acid desaturation, through its interaction with cytochrome b5.
• CYB5R3 localizes to the endoplasmic reticulum membrane, mitochondrial outer membrane, and cytoplasm, reflecting its multifunctional roles.
• It is expressed at late stages of erythroid maturation, suggesting an important function in red blood cell metabolism.
• Post-translational modifications include acetylation, myristoylation, and phosphorylation, which may regulate its membrane association and activity.
• Mutations in CYB5R3 are associated with congenital methemoglobinemia, highlighting its clinical significance.
• The protein has a calculated molecular weight of approximately 34 kDa (34235 Da), consistent with the observed band in western blotting.

Experimental Guidance and Technical Tips

• The immunogen spans amino acids 125–334 of human CYB5R3 (NP_001165131.1), a region conserved across typical isoforms; verify cross-reactivity in systems beyond human, mouse, or rat if required.
• In western blotting, expect a primary band around 34 kDa; alternative splicing or PTMs may yield additional bands, so validate in the relevant cell or tissue lysate.
• For immunofluorescence/ICC, the antibody can detect CYB5R3 in the ER and mitochondria, producing a reticular and punctate cytoplasmic pattern; include organelle markers for colocalization studies.
• For ELISA, titrate the antibody and use appropriate positive controls; pairing with a matched detection antibody is recommended for sandwich assays.
• Store the antibody at the recommended temperature (e.g., -20°C) upon receipt and minimize freeze-thaw cycles to preserve activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, technical services, and consulting for every development stage. For CYB5R3-related research in metabolic disorders or erythroid biology, we can source validated antibody pairs, recombinant proteins, and ancillary reagents, ensuring lot-to-lot consistency and scalability. Leverage our one-stop platform to accelerate your assay development from concept to clinic.