Anti-CYB5R3 Monoclonal Antibody for WB and ELISA - P00387

Core Product Specifications and Parameters

Biological Background: CYB5R3 (NADH-cytochrome b5 reductase 3) Function and Localization

• Also known as Diaphorase-1 (DIA1), this protein belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
• Catalyzes the reduction of two molecules of cytochrome b5 using NADH as the electron donor, participating in electron transport and fatty acid desaturation.
• Localizes to the endoplasmic reticulum membrane, mitochondrial outer membrane, and cytoplasm.
• Expressed at late stages of erythroid maturation, indicating a role in red blood cell development.
• Functions in cholesterol biosynthesis and lipid metabolism pathways.
• Contains FAD and NAD binding sites; classified as an oxidoreductase.
• Post-translational modifications include acetylation, phosphorylation, and myristoylation.
• Associated with disease variants according to UniProt keywords.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 1–100 of human CYB5R3; therefore, the antibody may recognize epitopes in the N-terminal region.
• Predicted molecular weight is approximately 34 kDa; use appropriate gel percentage and markers for WB.
• For WB, consider using lysates from human and rat cell lines that endogenously express CYB5R3.
• In ELISA, ensure the antigen coating matches the immunogen region or native protein conformation.
• Validate detection in relevant biological samples, such as erythroid cells or liver tissue, where CYB5R3 is functionally significant.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio connects diagnostic manufacturers, laboratories, and research institutes with high-quality IVD raw materials for targets involved in metabolic and redox biology. We offer validated monoclonal antibodies, optimized antibody pairs, and ancillary reagents to support your assay development for NADH-cytochrome b5 reductase 3 and related pathways. From concept to clinic, our sourcing network helps you secure rare-target raw materials with consistent batch-to-batch performance.