Anti-CYB5R1 Polyclonal Antibody for WB, IHC-P, ELISA - Q9UHQ9

Core Product Specifications and Parameters

Biological Background: CYB5R1 Function and Localization

• CYB5R1 is a 34 kDa transmembrane protein that belongs to the flavoprotein pyridine nucleotide cytochrome reductase family (UniProt Q9UHQ9).
• It functions as an NADH-cytochrome b5 reductase, catalyzing the transfer of electrons from NADH to two molecules of cytochrome b5.
• The enzyme is involved in lipid biosynthesis and metabolism, including the biosynthesis of steroids and sterols.
• CYB5R1 contains FAD as a cofactor and possesses oxidoreductase activity, consistent with its role in electron transport.
• It is localized to cellular membranes, consistent with the presence of a predicted transmembrane helix.
• The protein is widely expressed across human tissues, suggesting a housekeeping role.
• Alternative names include Humb5R2 and NQO3A2, reflecting its relationship to the quinone oxidoreductase family.

Experimental Guidance and Technical Tips

• For Western blotting, a predicted molecular weight of 34 kDa can be used for band identification; validate with appropriate positive and negative controls.
• In immunohistochemistry (paraffin), consider antigen retrieval methods due to the membrane localization of CYB5R1.
• As a polyclonal antibody raised against the full-length recombinant protein, it may detect multiple isoforms or post-translationally modified forms; verify specificity in your sample system.
• For ELISA applications, optimize coating and detection conditions using purified recombinant CYB5R1 as standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for diagnostic manufacturers and research laboratories, offering one-stop access to high-quality IVD raw materials. For targets such as CYB5R1—involved in lipid metabolism, steroid biosynthesis, and electron transport—we supply validated polyclonal antibodies, matched antibody pairs, and bulk ancillary reagents to support assay development from concept to clinic. Our streamlined procurement also covers rare-target sourcing and tailored technical consultation to accelerate your IVD projects.